John Wiley & Sons, Ltd.

Bisphenol A reduces fertilizing ability and motility by compromising mitochondrial function of sperm

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Bisphenol A acts as an endocrine disruptor, affects animal reproductive success in vivo, and affects sperm functions in vitro at environmentally relevant concentrations leading to reduction in sperm motility and fertilizing ability in fish. The effect of in vitro BPA on avian sperm functions has not been explored. We tested the effect of environmentally relevant concentrations of BPA (0, 0.18, 0.37 and 0.74 mM) on sperm functions in chicken in vitro. Sperm were exposed to concentrations of BPA for 30 min, and analyzed for motility, fertilizing ability, percent live sperm and Δψm. Results revealed that BPA significantly decreased motility, fertilizing ability, % live sperm count and sperm Δψm at concentration of 0.74 mM BPA. Sperm motility was positively correlated with fertility (r = 0.73, P≤0.01), % live sperm (r = 0.64, P≤0.01) and high Δψm (r = 0.44, P≤0.01). A dose and time dependent effect of BPA was observed on sperm motility at all the BPA concentrations. However, sperm's fertilizing ability was unaffected in low BPA concentration (0.18 and 0.37 mM). Significantly higher percentage of moribund sperm was observed at 0.37 and 0.74 mM BPA compared to 0.18 mM BPA, negative control and vehicle control. The present study confirms that environmentally relevant concentrations of BPA are capable in compromising sperm functions, leading to reduction in fertilizing ability of chicken sperm. This article is protected by copyright. All rights reserved

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