MATRIX: Apple Juice
RANGE OF DETECTION: 0.1 to 30 ppm
MATERIALS: Captan RaPID AssayÒ Kit and Sample Diluent. Reagents: methanol (pesticide grade) and distilled or deionized water. Equipment: vacuum manifold, stopcocks, BAKERBONDÔ spe octadecyl (C18) 500mg extraction column (cat. no.# 7020-03).
CONCENTRATION PROCEDURE: Condition C18 column with 12 mL methanol, followed by 12 mL water.
DO NOT ALLOW COLUMN TO RUN DRY.
Apply 15 mL apple juice sample to column. Adjust flow rate to 7.5 mL/min or less.
Wash column with 6 mL water. Dry under vacuum for 3 minutes. Elute captan frocmol umn
with three aliquots of 1 mL each of methaonl. Adjust final volume to exactly 3 mL with
methanol. Vortex to mix thoroughly.
ANALYSIS: Dilute an aliquot of the methanoel luate 1:50 in 'cold' (4-8oC) Captan Sample Diluent (0.2 mL eluate + 9.8 mL diluent). Analyze the diluted extract according to the package insert for the Captan RaPID Assay.