John Wiley & Sons, Ltd.

DNA damage and genetic methylation changes caused by CD in Arabidopsis thaliana seedlings

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Amplified Fragment Length Polymorphism (AFLP) and Methylation Sensitive Amplification Polymorphism (MASP) techniques are sensitive to DNA damage and genetic methylation respectively. In present experiment, Arabidopsis thaliana which cultured by 0 (CK), 0.5, 1.5, 5.0 mg/L Cd2+ for 16d was chosen to analyze the DNA damage and methylation changes caused by cadmium (Cd) using these two methods. The DNA was amplified by 14 AFLP primer‐pairs and 13 MSAP primer combinations. In AFLP experiment, 62 polymorphic sites were found in the patterns of 11 primer combinations and a total of 1116 fragments were gotten in these patterns. There were no polymorphic bands in the remaining three pairs. The proportion of polymorphic sites in 0.5 mg/L Cd2+ and 5.0 mg/L Cd2+ treatments were significantly different. Seven polymorphic fragments were then separated and sequenced successfully, which included 6 nucleobases substitution and 1 nucleobase deletion. Similarly, in MSAP experiment, MSAP% and the number of Demethylated type bands were unchanged after Cd treated, but the number of Methylated type bands was increased significantly in 5.0 mg/L Cd2+ treatment, which may be associated with the AFLP results. The polymorphic bands were also sequenced and found the functions of their homologous genes. The DNA damage and methylation changes may be the primary cause of some pathology changes in plants caused by Cd. This article is protected by copyright. All rights reserved

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