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Efficacy of selected probiotic cultures to inhibit Enterobacter sakazakii ATCC 12868 in model reconstituted dairy products

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Enterobacter sakazakii
ATCC 12868 (
ES
) is a very dangerous opportunistic organism likely to contaminate post-pasteurised dry milk products with a high mortality rate (40–80%). This study evaluated the antimicrobial efficacy of probiotics against
ES
in reconstituted dried infant formula. Initial study at 30ºC showed that
Enterococcus faecium
M-74 (
EF
) to exhibit the strongest inhibition when compared with
Lactobacillus acidophilus
74-2 (
LA
) and
Pediococcus acidilacticii
(
PA
). In later study, initial populations of
ES
and
EF
were ~ 10
2
CFU/ml and 10
9
CFU/ml in infant formula, respectively. At 12h,
ES
in controls at 30 and 35ºC increased to 6.54 and 7.95 log10 CFU/ml, respectively. At 12 hours,
ES
in formula (30ºC) with
EF
decreased to 0.52 log10 CFU/ml and was undetected (< 1.0 CFU/ml) at 35ºC.
EF
rapidly reduce the pH of the infant formula below pH 5.0 in the first four hours of fermentation at 35ºC, more than at 30ºC. The inhibitory effect of
EF
may be due to the acidification of the formula.

Keywords: probiotic, Enterobacter sakazakii, ES, Lactobacillus acidophilus 74-2, LA, Pediococcus acidilacticii, PA, Enterococcus faecium M-74, EF, infant formula, food pathogen, pathogen inhibition

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