Efficacy of selected probiotic cultures to inhibit Enterobacter sakazakii ATCC 12868 in model reconstituted dairy products
ATCC 12868 (
) is a very dangerous opportunistic organism likely to contaminate post-pasteurised dry milk products with a high mortality rate (40–80%). This study evaluated the antimicrobial efficacy of probiotics against
in reconstituted dried infant formula. Initial study at 30ºC showed that
) to exhibit the strongest inhibition when compared with
). In later study, initial populations of
were ~ 10
CFU/ml and 10
CFU/ml in infant formula, respectively. At 12h,
in controls at 30 and 35ºC increased to 6.54 and 7.95 log10 CFU/ml, respectively. At 12 hours,
in formula (30ºC) with
decreased to 0.52 log10 CFU/ml and was undetected (< 1.0 CFU/ml) at 35ºC.
rapidly reduce the pH of the infant formula below pH 5.0 in the first four hours of fermentation at 35ºC, more than at 30ºC. The inhibitory effect of
may be due to the acidification of the formula.
Keywords: probiotic, Enterobacter sakazakii, ES, Lactobacillus acidophilus 74-2, LA, Pediococcus acidilacticii, PA, Enterococcus faecium M-74, EF, infant formula, food pathogen, pathogen inhibition