John Wiley & Sons, Ltd.

Identification of compounds in heavy fuel oil that are chronically toxic to rainbow trout embryos by effects‐driven chemical fractionation

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This research isolated and identified compounds in heavy fuel oil 7102 (HFO 7102) that are bioavailable and chronically toxic to rainbow trout embryos (Oncorhynchus mykiss). An effects‐driven chemical fractionation combined the chemical separation of oil with toxicity testing and chemical analyses of each fraction to identify the major classes of compounds associated with embryo toxicity. Toxicity was assessed with two exposure methods, a high energy chemical dispersion of oil in water, which included oil droplets in test solutions, and water accommodated fractions produced by oiled gravel desorption columns, which did not contain visible oil droplets. Fractions of HFO with high concentrations of naphthalenes, alkanes, asphaltenes and resins were non‐toxic to embryos over the range of concentrations tested. In contrast, fractions enriched with 3 to 4‐ringed alkyl polycyclic aromatic hydrocarbons (PAH) were embryotoxic, consistent with published studies of crude oils and individual alkyl PAH. The rank order of fraction toxicity did not vary between the exposure methods, and was consistent with their PAH content; fractions with higher molecular weight alkyl PAH were most toxic. Exposure of juvenile trout to most fractions of HFO induced higher activities of cytochrome P450 enzymes, with a rank order of potency that varied with exposure method and differed somewhat from that of embryotoxicity. Induction reflected the bioavailability of PAH, but did not accurately predict embryotoxicity. Environ Toxicol Chem © 2013 SETAC

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