Inactivation of rotavirus in water by copper pot

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The objective of this study was to evaluate the use of copper pots for inactivation of rotavirus present in water. Distilled water was inoculated with rotavirus (2.2 × 107 plaque-forming units (PFU)/mL) and stored overnight (16 hours) at room temperature in copper pots (test) and in glass bottles (controls). The viable count of infectious virus was tested using a plaque assay on MA 104 monkey kidney cell lines. No plaques were recovered from the water stored in copper pots. On the other hand, over 106PFU/mL of virus was recovered from water stored in controls. The copper leached into the water was at a concentration of 447.25 ± 4.78 ppb, which is well within the safety limits prescribed by the World Health Organization (2,000 ppb). The copper pot has the potential to be used as a point-of-use household water purification system, especially against waterborne pathogens such as rotavirus, which is the cause of 22% of diarrhoea hospitalizations in children less than 5 years of age in developing countries.

Keywords: ayurveda, copper, diarrhoea, rotavirus, water

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