Keywords: atrazine, catfish, dissolved oxygen, herbicides, matrix solid-phase dispersion (MSPD), triazines, toxicity
Matrix solid phase dispersion extraction of triazines from catfish tissues; examination of the effects of temperature and dissolved oxygen on the toxicity of atrazine
A matrix solid-phase dispersion (MSPD) method to analyse the triazine herbicides atrazine, cyanazine, metribuzin and simazine in catfish muscle and liver is presented. An examination of the extract obtained in an immunoassay for triazines is also reported. The method is applied to the analysis of atrazine levels in catfish exposed to increasing levels of atrazine to determine toxicity to this species. The synergistic effects of atrazine concentration with increased water temperature and decreasing dissolved oxygen content were also examined. Potentiation of atrazine's toxicity was observed for changes in both environmental conditions, indicating that increases in temperature and/or lower dissolved oxygen content, commonly observed in certain regions of the world, could greatly enhance the toxicity of atrazine to aquatic species.