John Wiley & Sons, Ltd.

Morphological and transcriptional responses of Lycopersicon esculentum to hexavalent chromium in agricultural soil

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The carcinogenic, teratogenic and mutagenic effects of hexavalent chromium [Cr(VI)] to living organisms through food chain raise the immediate need to assess the potential toxicological impacts of Cr(VI) on human health. Therefore, the concentration‐dependent responses of 12 Cr(VI)‐responsive genes selected from the high‐throughput Lycopersicon esculentum cDNA microarray were examined at different Cr concentrations. Results indicated that most of the genes were differentially expressed as from 0.1 mg Cr/kg soil, while the lowest observable adverse effect concentration (LOAEC) of Cr(VI) were 1.6, 6.4, 3.2 and 0.4 mg Cr/kg soil for seed germination, root elongation, root biomass and root morphology, respectively, implying that the transcriptional method was more sensitive than the traditional method in detecting Cr(VI) toxicity. Dose‐dependent responses were observed for the relative expression of expansin (P = 0.778), probable chalcone‐flavonone isomerase 3 (P = ‐0.496) and 12S seed storage protein CRD (P = ‐0.614), therefore, we propose the three genes as putative biomarkers in Cr(VI)‐contaminated soil. This article is protected by copyright. All rights reserved

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