The Ty1 long terminal repeat (LTR) retrotransposon of Saccharomyces cerevisiae is a powerful model to understand the activation of transposable elements by stress and their impact on genome expression. We previously discovered that Ty1 transcription is activated under conditions of severe adenine starvation. The mechanism of activation is independent of the Bas1 transcriptional activator of the de novo AMP biosynthesis pathway and probably involves chromatin remodeling at the Ty1 promoter. Here, we show that the 5' LTR has a weak transcriptional activity and is sufficient for the activation by severe adenine starvation. Furthermore, we demonstrate that Ty1 insertions that bring Ty1 promoter sequences into the vicinity of a reporter gene confer adenine starvation regulation on it. We provide evidence that similar coactivation of genes adjacent to Ty1 sequences occurs naturally in the yeast genome, indicating that Ty1 insertions can mediate transcriptional control of yeast gene expression under conditions of severe adenine starvation. Finally, the transcription pattern of genes adjacent to Ty1 insertions suggests that severe adenine starvation facilitates the initiation of transcription at alternative sites, partly located in the 5' LTR. We propose that Ty1-driven transcription of coding and noncoding sequences could regulate yeast gene expression in response to stress.
- American Society for Microbiology
- Remodeling yeast gene transcription by activating the ty1 long ...
Housekeeping at Work – Don’t Sweep It Under the Rug
What’s at Stake? Housekeeping at work is about much more than cleaning windows and sweeping up dust bunnies. Poor housekeeping can cause injuries, slow down production, dampen morale, start fires and cause catastrophic explosions of combustible dusts. What’s the Danger? A messy workplace not only affects workers’ safety and morale, it makes a bad impression on potential and current customers and regulatory officers. How much harder do you think an inspector will look at your work area if...
Government flooding plans come under attack from recent report
A recent report from MPs has highlighted inadequacies over the current plans for flooding by the government, suggesting that the Environmental Agency be stripped of their current responsibilities and replaced by a dedicated flooding authority. As we head into another flooding season, it is disheartening and worrying to see that the current solutions are found to be lacking. Read our new LinkedIn article to find out more and see our take on the new report.
Critical Operations Go Under Cover
For a number of years, composting facilities have been using fabric structures to house finished compost screening, soil blending and product storage. They’ve also employed the structures to cover or fully enclose feedstock receiving and mixing. And some have gone as far as to put active composting operations inside fabric buildings. “More and more facilities are putting in fabric structures for a number of different reasons,” notes Bill Kish, who directs business development activities at...
Manage Your Farm Inventory with Agrivi Software
Every farmer, whether on a small family farm or with large farm production, has an inventory of all his farm materials; crop seeds, fertilizers, pesticides, fuel, harvested crops and other farm items. Whatever the size of his production, he uses those materials on fields and thus has the necessity to keep records of their inventory level. In farm management, inventory plays a key role. It’s important for farmers to know at any moment the exact amount of items. Farmers can achieve that with Agrivi farm...
Mexico’s first commercial waste to energy facility to supply alternative fuel under agreement
Mexico’s first Waste to Energy plant is up and running in Hermosillo, Sonora. The facility began to take shape two years ago, when total waste management specialist Promotora Ambiental SAB de CV (PASA) set out to enter the WtE market. With more than 6,500 employees, a fleet of over 2,000 vehicles and 43 waste management facilities in Mexico, this environmental company handles 25,000 tonnes of waste every day. But acknowledging the growing pressures being placed on the country’s resource infrastructure...