The Ty1 long terminal repeat (LTR) retrotransposon of Saccharomyces cerevisiae is a powerful model to understand the activation of transposable elements by stress and their impact on genome expression. We previously discovered that Ty1 transcription is activated under conditions of severe adenine starvation. The mechanism of activation is independent of the Bas1 transcriptional activator of the de novo AMP biosynthesis pathway and probably involves chromatin remodeling at the Ty1 promoter. Here, we show that the 5' LTR has a weak transcriptional activity and is sufficient for the activation by severe adenine starvation. Furthermore, we demonstrate that Ty1 insertions that bring Ty1 promoter sequences into the vicinity of a reporter gene confer adenine starvation regulation on it. We provide evidence that similar coactivation of genes adjacent to Ty1 sequences occurs naturally in the yeast genome, indicating that Ty1 insertions can mediate transcriptional control of yeast gene expression under conditions of severe adenine starvation. Finally, the transcription pattern of genes adjacent to Ty1 insertions suggests that severe adenine starvation facilitates the initiation of transcription at alternative sites, partly located in the 5' LTR. We propose that Ty1-driven transcription of coding and noncoding sequences could regulate yeast gene expression in response to stress.
- American Society for Microbiology
- Remodeling yeast gene transcription by activating the ty1 long ...
Modification and characterization of microwave assisted N doped TiO2 – a photodegradation study ...
A commercially available TiO2 (P25) was modified to produce nitrogen (N) doped TiO2 prepared by mixing TiO2 powder with various amounts of urea as N precursor using a microwave irradiation heating technique. X-ray photoelectron spectroscopy analysis showed that N chemically bonded with TiO2 produces Ti-N. The measured band gap energy (Eg) of the modified sample was ca. 2.9 eV as detected by UV-Vis/DRS. The optimum modified N doped TiO2 denoted as U3-800 prepared at 230 °C showed an active visible light photoactivit...
Housekeeping at Work – Don’t Sweep It Under the Rug
What’s at Stake? Housekeeping at work is about much more than cleaning windows and sweeping up dust bunnies. Poor housekeeping can cause injuries, slow down production, dampen morale, start fires and cause catastrophic explosions of combustible dusts. What’s the Danger? A messy workplace not only affects workers’ safety and morale, it makes a bad impression on potential and current customers and regulatory officers. How much harder do you think an inspector will look at your work area if...
Water transparency distribution under varied currents in the largest river-connected lake of China
Water transparency is an important ecological indicator for shallow lakes. The largest shallow lake, Poyang Lake, as well as the most typical river-connected lake in China was selected as the research area. In view of the complicated water-sediment conditions induced by its frequent water exchange with external rivers, the dominant factors driving water transparency were determined against the field investigated data from 2003 to 2013 and a specific driving function was established. A numerical model coupling...
Assessing buried infrastructure deep under buried infrastructure
In preparing for its water future, the Region of Peel (Peel) adopts a unique assessment strategy for a newly constructed potable water transmission main that extends deep underground through the heart of Peel Region. The effort is paying off, with Peel decision makers gaining a better understanding of this pipeline as it comes into service.
Government flooding plans come under attack from recent report
A recent report from MPs has highlighted inadequacies over the current plans for flooding by the government, suggesting that the Environmental Agency be stripped of their current responsibilities and replaced by a dedicated flooding authority. As we head into another flooding season, it is disheartening and worrying to see that the current solutions are found to be lacking. Read our new LinkedIn article to find out more and see our take on the new report.