Selenite supplementation reduces arsenate uptake greater than phosphate but compromises the phosphate level and physiological performance in hydroponically grown Oryza sativa L.
The study evaluates the reduction of As(V) uptake in rice seedlings through individual and combined supplementation of PO43‐, Se(IV) under hydroponic condition. The toxic response in seedlings receiving As(V), manifested as inhibition in physiological parameters such as WUE, Gs, A, E, qp and ETR along with the growth. Arsenic accumulation significantly decreased with Se(IV) treatment (0.5, 1 and 2 µg ml−1) in a dose dependent manner (20%, 35%, 53%, respectively), however, it compromised the PO43‐ level and physiological performance. The lower level of Se(IV) (0.5 µg ml−1) was relatively beneficial in terms of reduction in As accumulation than the higher level of Se(IV) (2 µg ml−1) which was rather toxic. Further, decrease in As uptake, replenished the level of PO43‐ and physiological performance were observed in the seedlings treated with As + Se + P, compared to the As + Se. However, supplementation of only PO43‐ (10 and 20 µg ml−1) along with As(V), was less effective in reduction of As accumulation, as compared to As + Se. The seedlings receiving As + Se + P also exhibited lower TBARS and EC levels as compared to the both As + Se and As + P. Among all the treatments, the activity of antioxidant enzymes was highest in plants treated with As + Se + P. Hence, the higher antioxidant enzymes activity in As + Se + P along with lower levels of TBARS, H2O2 and As accumulation, are attributed to the competitive reduction in As uptake in presence of Se(IV) and PO43‐. This article is protected by copyright. All rights reserved