John Wiley & Sons, Ltd.

TBECH‐mediated steroid hormone receptor activation and gene regulation in chicken LMH cells

0
The incorporation of brominated flame retardants into industrial and household appliances has increased their occurrence in the environment resulting in deleterious effects on wildlife. With the increasing restraints on available compounds there has been a shift to using BFRs that has seen the production of alternative BFRs such as 1,2‐dibromo‐4‐(1,2 dibromoethyl) cyclohexane (TBECH). TBECH has been detected in the environment and in previous in silico and in vitro studies we have shown that TBECH can activate both the human androgen receptor (hAR) and the zebrafish AR (zAR) suggesting that it is a potential endocrine disruptor. The present study was aimed at determining the interaction of TBECH with the chicken AR (cAR). TBECH bound to cAR but in vitro activation assay studies using the chicken LMH cell line showed it only had a potency of 15% compared to testosterone (T). Sequence difference between AR from different species may contribute to the different responses to TBECH. Further qRT‐PCR analysis showed that TBECH interacted with and alters the expression of both thyroid receptors (TR) and estrogen receptors (ER). In addition the qRT‐PCR analysis also showed that TBECH altered the transcription pattern of genes involved in inflammatory, apoptotic, proliferative, DNA methylation and drug metabolizing pathways. This demonstrates that TBECH apart from activating cAR can also influence multiple biological pathways in chicken. Environ Toxicol Chem © 2013 SETAC

Customer comments

No comments were found for TBECH‐mediated steroid hormone receptor activation and gene regulation in chicken LMH cells. Be the first to comment!