The major fungal pathogen of humans, Aspergillus fumigatus, lacks a defined sexual cycle, although the presence of genes encoding putative mating type idiomorphs and regulators of Aspergillus sexual development heightens the potential for cryptic sexuality in this deuteromycete. To test the functionality of these genetic determinants, we transferred the alpha box-encoding mat1-1 idiomorph from an A. fumigatus isolate to the homothallic fertile species Aspergillus nidulans. Abundant formation of fruiting bodies (cleistothecia) containing viable ascospores establishes functionality of this mating type gene product in the transgenic strain. Using a similar approach, we also established that the conserved transcriptional regulator from A. fumigatus, the nsdD gene product, can act as a functional, positively acting factor for A. nidulans cleistothecium development; moreover, high-level expression of NsdD in the endogenous host A. fumigatus profoundly alters hyphal development by triggering the formation of coiled hyphae. Our findings demonstrate that the presumably asexual pathogen A. fumigatus encodes functional regulators of mating and sexual development, thereby potentiating the case for cryptic sexuality in this fungal pathogen.
- American Society for Microbiology
- The asexual pathogen aspergillus fumigatus expresses functional ...
On site at Northdown Orchard Organic Farm
On Saturday 15th ofOctober we were on site with Mike and the team at Northdown Orchard, an existing customer who was kind enough to show us around the farmand let us snap somegreat photos at his site in exchange for a morning demonstrating our latest stone burier on his tractor. About Northdown Orchard The organic farm is a family run affair and sits on a site of 12 acres. Mike and his ever growing team produce salads, herbs and vegetables throughout the year in the farmed fields and polytunnels. The farm...
UncerTZ Consortium - Time to Depth Conversion Evolved
Near the end of 2015 Geovariances committed itself into building a new, sophisticated tool for Time to Depth conversion in Reservoir Characterization. The challenges petitioned by the consortium partners were not easy. It included features such as: more choices in conversion (and less of a “black box”), uncertainty characterization, inclusion of faults in the conversion procedure, easy to use software, fast to operate so that it can be included in a bigger workflow, among others. Geovariances...
Chicken Manure Management, Recycling, and Composting
Chicken Manure Fertilizer Manufacturing Process If you have chickens, you will have manure. Fortunately, chicken manure is among the most prized of manures. Fresh chicken manure is considered a “hot” manure, which is unsuitable for immediate use. Chicken manure needs to be composted and aged at least two to three months before you add it to your garden. If you don’t wait that long, it will burn your plants. Manure is a good source of organic material for composting. Not all manures are the...
WRIB 2016: “Diversity of Platforms and Approaches Needed for Bioanalysts”
Held April 18–22 in Lake Buena Vista, Florida, WRIB 2016 was well attended this year, with about 700 attendees. This 10th Workshop on Recent Issues in Bioanalysis, where regulators and professionals representing pharma/biopharma, biotech, CRO organizations gather to discuss challenges in method development and emerging approaches, proved as fruitful as years past. Here are a few high-level takeaways from the main talks: It’s about the and, not the or: Both ligand binding assays (LBAs) and analysis...
Recycle plastic HDPE Crate Moulds
According the appliance, usually we classify the plastic crate into following: Industry large plastic crates/box/container mouldsAgriculture plastic crates moulds for vegetables,fruits, fish...Plastic bottle crate moulds for Coca cola, milk...Plastic Crates moulds for the Bakery IndustryPlastic Crates moulds for the Dairy IndustryPlastic Crates moulds for Grocery DistributionPlastic Crates molds for the Fishing IndustryBased on these plastic crates moulds manufacturing experience, we established our own standard...