John Wiley & Sons, Ltd.

The effect of cuo nps on ros and the cell cycle gene expression in roots of rice

To evaluate the effect of CuO NPs on root growth, root ROS production and the expression of two genes (OsCDC2 and OsCYCD) associated with root growth of Oryza sativa (rice), the rice roots were treated with 5 mg/L CuO NPs suspension, 5 mg/L CuO BPs suspension, 0.27 mg/LCuSO4.5H2Osolution and distilled water respectively. Results indicated that CuO NPs and Cu2+ severely inhibited the elongation and biomass of rice roots after 72 h exposure. The DCFH‐DA dyeing exhibited that in these four different groups, the fluorescence primarily located in the meristem zone, which demonstrated that meristem zone was the place where ROS was primarily generated. Also, significant increase of ROS was detected in the meristem zone of roots treated with the CuO NPs suspension and CuSO4.5H2Osolution. The expression level of OsCDC2 and OsCYCD had also been greatly influenced by them. But the impact of Cu2+ on these two genes was smaller than that of CuO NPs. The copper content in roots of rice with CuO NPs treatment was greatly higher than that of other treatments, which indicated that CuO NPs may have been absorbed into the tissues of roots. Collectively, the present study suggests that growth inhibition, more ROS production and gene expression inhibition may not only be caused by the ions themselves, the nanoparticles also played an important role. This article is protected by copyright. All rights reserved

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