Bacteria from the genus Streptomyces are among the most complex of all prokaryotes; not only do they grow as a complex mycelium, they also differentiate to form aerial hyphae before developing further to form spore chains. This developmental heterogeneity of streptomycete microcolonies makes studying the dynamic processes that contribute to growth and development a challenging procedure. As a result, in order to study the mechanisms that underpin streptomycete growth, we have developed a system for studying hyphal extension, protein trafficking, and sporulation by time-lapse microscopy. Through the use of time-lapse microscopy we have demonstrated that Streptomyces coelicolor germ tubes undergo a temporary arrest in their growth when in close proximity to sibling extension sites. Following germination, in this system, hyphae extended at a rate of ~20 µm h–1, which was not significantly different from the rate at which the apical ring of the cytokinetic protein FtsZ progressed along extending hyphae through a spiraling movement. Although we were able to generate movies for streptomycete sporulation, we were unable to do so for either the erection of aerial hyphae or the early stages of sporulation. Despite this, it was possible to demonstrate an arrest of aerial hyphal development that we suggest is through the depolymerization of FtsZ-enhanced green fluorescent protein (GFP). Consequently, the imaging system reported here provides a system that allows the dynamic movement of GFP-tagged proteins involved in growth and development of S. coelicolor to be tracked and their role in cytokinesis to be characterized during the streptomycete life cycle.
- American Society for Microbiology
- Time-lapse microscopy of streptomyces coelicolor growth and ...
EPA Convenes Hearing On Proposed Renewables Enhancement and Growth Support Rule
On December 6, 2016, EPA held a hearing on its proposed Renewables Enhancement and Growth Support rule. The proposed rule would update the regulatory structure to allow biofuel producers to process and convert biomass at different facilities, update fuel regulations to allow for more high-ethanol fuel blends in flex fuel vehicles, and permit cellulosic biofuels to be produced from new feedstock sources. Testimony provided by Growth Energy and the Renewable Fuels Association (RFA) focused on the impact of the...
EBS upgrades its Bacterial Acceleration Chambers to further enhance bacterial growth
Continual innovation is an important component of the EBS core value called “Simple yet Profound.” This core value is rooted in the fact that meaningfully applied technology doesn’t have to be rocket science. Over the years, EBS has pursued the cutting edge of wastewater bioaugmentation.We relentlessly “peel the onion” in our pursuit for answers and the application of “applying” our newfound knowledge to assist clients in their goal to run their wastewater treatment plant...
EPA Announces Public Hearing For Proposed Renewables Enhancement and Growth Support Rule
On November 10, 2016, the U.S. Environmental Protection Agency (EPA) issued a notice in the Federal Register for a public hearing for the proposed “Renewables Enhancement and Growth Support Rule.” The proposed rule, available on EPA’s website, was signed by EPA’s Administrator on October 3, 2016, but has not yet been published in the Federal Register. This proposed rule will enhance the renewable fuel standard (RFS) program as well as other fuel regulations to support the growth of renewable...
EPA Releases Proposed Rule On Renewables Enhancement And Growth Support
On October 3, 2016, the U.S. Environmental Protection Agency (EPA) released the proposed Renewables Enhancement and Growth Support Rule. This proposed rule includes suggestions to improve the Renewable Fuel Standard (RFS) program and related pro-renewable fuel regulations. Updated regulations included in the proposed rule include allowing biofuel producers to process feedstock and then convert the material into fuels at different facilities, and an expansion to the availability of high-ethanol fuel blends. The...
Enhanced sonochemical degradation of tetracycline by sulfate radicals
Tetracyclines (TCs) are widely used antibiotics in human and veterinary medicine and as growth promoters in the world. Sulfate radical-based advanced oxidation processes have been of great interest due to the high oxidizing potential of sulfate radical to degrade refractory organic pollutants in aqueous solution. In this study, the degradation of antibiotic TC in aqueous solution by silver-activated persulfate (Na2S2O8) in the presence of ultrasound irradiation under various conditions has been investigated. The...