John Wiley & Sons, Ltd.

Use of gene expression data to determine effects on gonad phenotype in Japanese medaka after exposure to trenbolone or estradiol

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Various aquatic bioassays using one of several fish species have been developed or are in the process of being developed by organizations like the US Environmental Protection Agency and the Office of Economic Cooperation and Development for testing potential endocrine disrupting chemicals. Often these involve assessment of the gonad phenotype of individuals as a key endpoint that is inputted into a risk or hazard assessment. Typically, gonad phenotype is determined histologically, which involves specialized and time consuming techniques. The methods detailed here utilize an entirely different methodology, reverse transcription quantitative PCR, to determine the relative expression levels of four genes after exposure to either 17β‐estradiol or 17β‐trenbolone, and by extension, the effects of endocrine disrupting chemicals on the phenotypic status of the gonad. The four genes quantified, Sox9b, protamine, Fig1alpha, and ZPC1, are all involved in gonad development/maintenance in Japanese medaka (Oryzias latipes); this data is then inputted into a permutational MANOVA to determine whether significant differences exist between treatment groups. This information in conjunction with the sexual genotype, which can be determined in medaka, can be used to determine adverse effects of exposure to EDCs in a similar fashion to the histologically determined gonad phenotype. Environ. Toxicol. Chem. © 2013 SETAC

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