58 Articles found
American Society for Microbiology (ASM) Articles
-
Diverse phage-encoded toxins in a protective insect endosymbiont
The lysogenic bacteriophage APSE infects `Candidatus Hamiltonella defensa,` a facultative endosymbiont of aphids and other sap-feeding insects. This endosymbiont has established a beneficial association with aphids, increasing survivorship ...
-
Joint immobilization of plant growth-promoting bacteria and green microalgae in alginate beads as an experimental model for studying plant-bacterium interactions
A simple, quantitative experimental model, offering a convenient and basic approach to studies of plant-bacterium interactions, is proposed. This involves immobilizing a unicellular, freshwater microalga, a Chlorella species, serving as the ...
-
Translation initiation factor 2 mutant alters start codon selection independent of Met-tRNA binding
Selection of the AUG start codon for translation in eukaryotes is governed by codon-anticodon interactions between the initiator Met-tRNAiMet and the mRNA. Translation initiation factor 2 (eIF2) binds Met-tRNAiMet to the 40S ribosomal subunit, and ...
-
Phosphorylated ssk1 prevents unphosphorylated ssk1 from activating the ssk2 mitogen-activated protein kinase kinase kinase in the yeast high-osmolarity glycerol osmoregulatory pathway
In Saccharomyces cerevisiae, external high osmolarity activates the Hog1 mitogen-activated protein kinase (MAPK), which controls various aspects of osmoadaptation. Ssk1 is a homolog of bacterial two-component response regulators and activates the ...
-
The pentatricopeptide repeat protein ppr5 stabilizes a specific trna precursor in maize chloroplasts
Genes for pentatricopeptide repeat (PPR) proteins are found in all eukaryotic genomes analyzed but are particularly abundant in land plants. The majority of analyzed PPR proteins play a role in the processing or translation of organellar RNAs. Few ...
-
Nuclear export receptor xpo1/crm1 is physically and functionally linked to the spindle pole body in budding yeast
The spindle pole body (SPB) represents the microtubule organizing center in the budding yeast Saccharomyces cerevisiae. It is a highly structured organelle embedded in the nuclear membrane, which is required to anchor microtubules on both sides of ...
-
Remodeling yeast gene transcription by activating the ty1 long terminal repeat retrotransposon under severe adenine deficiency
The Ty1 long terminal repeat (LTR) retrotransposon of Saccharomyces cerevisiae is a powerful model to understand the activation of transposable elements by stress and their impact on genome expression. We previously discovered that Ty1 transcription ...
-
Salmonella enterica serovar enteritidis genes induced during oviduct colonization and egg contamination in laying hens
Salmonella enterica serovar Enteritidis is the predominant serovar associated with salmonellosis worldwide, which is in part due to its ability to contaminate the internal contents of the hen`s egg. It has been shown that S. enterica serovar ...
-
Gene transcription in lactarius quietus-quercus petraea ectomycorrhizas from a forest soil
Extracting fungal mRNA from ectomycorrhizas (ECMs) and forest soil samples for monitoring in situ metabolic activities is a significant challenge when studying the role of ECMs in biogeochemical cycles. A robust, simple, rapid, and effective ...
-
Comparative mycotoxin profiles of gibberella zeae populations from barley, wheat, potatoes, and sugar beets
Gibberella zeae is one of the most devastating pathogens of barley and wheat in the United States. The fungus also infects noncereal crops, such as potatoes and sugar beets, and the genetic relationships among barley, wheat, potato, and sugar ...
-
Nitric Oxide as a signaling factor to upregulate the death-specific protein in a marine diatom, skeletonema costatum, during blockage of electron flow in photosynthesis
To determine the physiological functions of a novel death-specific protein gene, Skeletonema costatum DSP-1 (ScDSP-1) in a marine diatom, Skeletonema costatum, the mRNA abundance of ScDSP-1 was measured in cultures subjected to light manipulation ...
-
Lactate and amino acid catabolism in the cheese-ripening yeast yarrowia lipolytica
The consumption of lactate and amino acids is very important for microbial development and/or aroma production during cheese ripening. A strain of Yarrowia lipolytica isolated from cheese was grown in a liquid medium containing lactate in the ...
-
Evidence for the role of calcineurin in morphogenesis and calcium homeostasis during mycelium-to-yeast dimorphism of paracoccidioides brasiliensis
Paracoccidioides brasiliensis is a dimorphic fungus that causes paracoccidioidomycosis, the most prevalent human deep mycosis in Latin America. The dimorphic transition from mycelium to yeast (M-Y) is triggered by a temperature shift from 25°C to ...
-
The crucial role of the pls1 tetraspanin during ascospore germination in podospora anserina provides an example of the convergent evolution of morphogenetic processes in fungal plant pathogens and saprobes{triangledown}
Pls1 tetraspanins were shown for some pathogenic fungi to be essential for appressorium-mediated penetration into their host plants. We show here that Podospora anserina, a saprobic fungus lacking appressorium, contains PaPls1, a gene ...
-
Autophosphorylation-induced degradation of the Pho85 cyclin Pcl5 is essential for response to amino acid limitation
Pho85 cyclins (Pcls), activators of the yeast cyclin-dependent kinase (CDK) Pho85, belong together with the p35 activator of mammalian CDK5 to a distinct structural cyclin class. Different Pcls target Pho85 to distinct substrates. Pcl5 targets Pho85 ...
-
Different genetic functions for the rpd3(l) and rpd3(s) complexes suggest competition between nua4 and rpd3(s)
Rpd3(L) and Rpd3(S) are distinct multisubunit complexes containing the Rpd3 histone deacetylase. Disruption of the GCN5 histone acetyltransferase gene shows a strong synthetic phenotype when combined with either an sds3 mutation affecting only the ...
-
The gluconeogenic enzyme fructose-1,6-bisphosphatase is dispensable for growth of the yeast yarrowia lipolytica in gluconeogenic substrates
The genes encoding gluconeogenic enzymes in the nonconventional yeast Yarrowia lipolytica were found to be differentially regulated. The expression of Y. lipolytica FBP1 (YlFBP1) encoding the key enzyme fructose-1,6-bisphosphatase was not ...
-
Oral administration of lactobacillus strains isolated from breast milk as an alternative for the treatment of infectious mastitis during lactation
In this study, 20 women with staphylococcal mastitis were randomly divided in two groups. Those in the probiotic group daily ingested 10 log10 CFU of Lactobacillus salivarius CECT5713 and the same quantity of Lactobacillus gasseri CECT5714 for 4 ...
-
Plasmid pcs1966, a new selection/counterselection tool for lactic acid bacterium strain construction based on the orop gene, encoding an orotate transporter from lactococcus lactis
In this paper we describe the new selection/counterselection vector pCS1966, which is suitable for both sequence-specific integration based on homologous recombination and integration in a bacteriophage attachment site. This plasmid harbors oroP, ...
-
Intracellular ph homeostasis plays a role in the tolerance of debaryomyces hansenii and candida zeylanoides to acidified nitrite
The effects of acidified-nitrite stress on the growth initiation and intracellular pH (pHi) of individual cells of Debaryomyces hansenii and Candida zeylanoides were investigated. Our results show that 200 µg/ml of nitrite caused pronounced growth ...