Measuring Ion Concentrations in Plant Tissue
- Aptisens Digital or Mobile Multiple Ion Kit
- Weighing scales
- Liquid measurement device e.g. graduated cylinder
- Bowl for stirring
- Spoon for stirring
- Filter paper / muslin
- Conical flask / vessel
- Aptisens multiple ion conditioning solution
- Aptisens Plant Tissue multiple ion calibration solutions
- Deionised water
This preparation can be applied to any plant tissue. Take a sample of lettuce and remove any soil but do not wash the plant tissue as doing so may reduce the number of ions in sample. Weigh this sample and record (say 100 g). Macerate the plant tissue in a blender and add a known amount of hot deionised water to the sample to extract the nutrients. For example, dilute the sample two-fold (200 mL), three-fold (300 mL) or five-fold (500 mL). Ensure all the plant tissue is covered with water and leave to sit for approximately 30 min with some stirring. The sample then needs to be filtered.
Set up a funnel with folded filter paper in a conical flask or any vessel that can hold a funnel. Pour the plant tissue and water into the funnel and allow the mixture to filter. Collect the filtrate.
Before use the multiple ion probe must be conditioned in Aptisens multiple ion conditioning solution. Ensure the correct calibration solutions for a three-point calibration are set up on the Mobile meter or MeterLink. Follow the instructions for calibration and proceed to take a sample reading when the calibration data is ‘very good’ or ‘good’ for all ions.
Place the probe in the plant tissue filtrate and ‘Take a Sample’ reading. The concentrations for each ion will be displayed in ppm or mmol / L.
Remember to multiply these concentrations by the dilution factor.
After taking your reading be sure to remove the sensors from the solution as quickly as possible, rinse with deionised water and replace the cap of the probe
By Aptisens based in Little Island, IRELAND.
Companies specialising in tissue culture multiply large numbers of plants in a short space of time by propagating plant parts and plant cells in artificial culture media. This is done in vitro under sterile conditions. Unlike with cuttings, the primary rooting often takes place in vitro too. Tissue-culture or in-vitro plants are nowadays used by thousands of growers. For some species, such as Lisianthus, orchids, lilies and various perennials, this is the most common form of propagation. A steadily increasing proportion of such plants now make their way to end users in Greenplugs or Aero NT plugs.
By Green Products B.V. based in Kampen, NETHERLANDS.
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