ERM-BF441, a series of certified reference materials for the quantification of DBN-09004-6 soya bean
30/05/2023 -- The EC-JRC produced a set of GMO reference materials certified for their mass fraction of DBN‑09004‑6 soya bean, enabling the accurate quantification of DBN‑09004‑6 soya bean in food and feed products by quantitative polymerase chain reaction (qPCR).
Beijing DaBeiNong Biotechnology Co., Ltd. (DBNBC) (Beijing, CN) has developed DBN‑09004‑6 soya bean (unique identifier code DBN‑Ø9ØØ4‑6). This transgenic soybean variety was genetically modified to express Agrobacterium tumefaciens CP4 EPSPS and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (PAT) proteins, which confer resistance and tolerance to glyphosate and glufosinate herbicides, respectively.
DBNBC asked the JRC to develop certified reference materials (CRMs) for DBN‑09004‑6 soya bean in compliance with EU Regulation No 1829/2003, as required for the authorisation of the GMO event for the EU's food and feed market. The resulting set of CRMs includes ERM-BF441a, ERM-BF441b, ERM-BF441c, ERM-BF441d, and ERM-BF441e, featuring varying levels of mass fractions of DBN‑09004‑6 soya bean. The concentration of DBN‑09004‑6 soya bean in ERM-BF441c and ERM-BF441d closely matches the 0.1 (m/m)% threshold for feed and 0.9% labelling threshold respectively, as laid down in EU’s GMO labelling legislation. ERM-BF441b has been designed to serve as a calibrant for the official qPCR method.
The CRMs represent a significant contribution towards ensuring the accuracy and reliability of GMO quantification measurements for DBN‑09004‑6 soya bean. They are indispensable tools for GMO testing and official control laboratories to ensure compliance with EU regulatory requirements.
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