Model M (PVM) - Suspicious Potato Plant Samples Test Strip
This test is intended to detect PVM in suspicious potato plant samples. PVM is a world-wide distributed virus. In potato, symptoms range from very slight to severe, depending on virus strain and potato variety. The virus causes various mottle, mosaic, crinkling and rolling symptoms in leaves, and stunting of shoot. The virus is transmitted both by aphids and mechanical means.
Specificity and sampling instruction
Mono- and polyclonal antibodies are used for this AgriStrip assay. This AgriStrip assay reacts specifically with PVM. No cross-reaction is observed with other potato viruses, such as PVA, PVS, PVX, PVY, and PLRV. Strongest coloration of testbands is obtained with an 1:60 (w/v) dilution of an extract of infected potato leaves.
Instructions for use
- Place approx. 0.1 g of leaf (corresponds to the size of 1 Euro coin or ≈ 5 cm2) into an extraction bag (Fig. 1*) and add 3 ml of AgriStrip extraction buffer A with a disposable pipette (1:30 w/v).
- Homogenize the tissue with a handheld homogenizer with a few movements for not more than 2-3 seconds (Fig. 2*).
- Transfer 2 drops of extract (Fig. 3*) and 2 drops of extraction buffer into a cuvette.
- Insert the end of the strip marked «sample» into the extract (Fig. 4*) and observe formation of colored bands.