Meizheng - Model HCM1115/HCM1150B - Biotin (VB7) Immunoaffinity Column
From Food Safety - Constituents
Biotin (VB7) Immunoaffinity Column with HPLC is a quantitative method for the detection of Biotin (VB7) in milk, milk powder etc. Samples are purified by the Column before quantitated by high performance liquid chromatography (HPLC). Immunoaffinity Column with HPLC can improve signal-noise ratio (SNR) and increase the accuracy of the detection results.
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Product Details
Product Name: Biotin (VB7) Immunoaffinity Column
- Catalog No.: HCM1115/HCM1150B
- Specification: 3mL 15/50T
- Storage Condition: 2-8℃
- Restriction: For research use only!
Summary
Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7).
It is composed of an ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.
Principle
The detection is based in antigen-antibody reaction. The antibody is bound in the column. Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the column bound with specific antibodies to Biotin (VB7). At this stage, the Biotin (VB7) binds to the antibody on the column. The column is then washed to rid the immunoaffinity column of impurities. By passing methanol through the column, the Biotin (VB7) is removed from the antibody. This methanol solution can then be injected into an HPLC system.
Kit Composition
Each kit contains Biotin (VB7) Immunoaffinity Columns of different specifications and one copy of Instruction for Use.