Mericon - DNA Bacteria Kit - For Extraction Of DNA From Bacteria After Enrichments
From Food Safety Testing - Sample Preparation Kits
The mericon food testing portfolio is a complete system of sample preparation and assay kits that meet the increasing demands of food research and monitoring. They provide streamlined solutions that are sensitive, accurate, and easy to use with a variety of starting materials.
-
Most popular related searches
The DNeasy mericon Food Kit, mericon DNA Bacteria ...
The DNeasy mericon Food Kit, mericon DNA Bacteria Kit, and mericon DNA Bacteria Plus Kit are designed to enable extraction of high-quality nucleic acids from a wide range of starting materials, such as pathogen enrichment cultures and a variety of raw and processed foods. The carryover of PCR inhibitors from these complex food matrices is minimized.
All three kits complement mericon PCR Assays for the detection of pathogens, genetically modified organisms, or identification of plant or animal matter in food, to create a streamlined workflow for efficient sample processing and reliable results.
For extraction of high-quality DNA from microbial enrichment culture and raw or processed foods
- Modified CTAB procedure ensures faster food sample processing
- Efficient purification of even fragmented DNA from processed foods
- Fast and efficient lysis of both Gram-negative and Gram-positive bacteria
- No carryover of PCR inhibitors from complex food matrices
The DNeasy mericon Food Kit and mericon DNA Bacteria Kits offer a streamlined sample preparation workflow that seamlessly integrates with real-time PCR assays testing for pathogens, genetically modified organisms, allergens, or animal and plant matter.
Principle
DNeasy mericon Food Kit
The DNeasy mericon Food Kit uses improved cetyltrimethylammonium bromide (CTAB) extraction of total cellular nucleic acids.
The nonionic detergent CTAB is widely used for efficient extraction of total cellular nucleic acids from a wide range of tissue types. Depending on the salt conditions, CTAB may complex with cellular nucleic acids (low-salt conditions) or complex with cellular inhibitors, such as polysaccharides, proteins, and plant metabolites (high-salt conditions; as found in the Food Lysis Buffer).
With fewer workflow steps than conventional CTAB protocols, up to 30 samples can be processed in 2.5 hours. DNA recovery with these kits is demonstrably high and efficient.
During production, food is subjected to heat, irradiation, high pressure, and changes in pH which lead to DNA degradation and fragmentation. The optimized chemistry of the DNeasy mericon Food Kit was developed to recover short DNA fragments (down to 100 bp) ensuring that even highly fragmented DNA is efficiently isolated and subsequently amplified in PCR reactions. With these features, extraction with the DNeasy mericon Food Kit is the first universally applicable extraction method that generates optimal and reliable results even when using strongly inhibitory, highly processed, fatty, acidic, high, or low DNA content foods. In addition, establishing multiple lysis procedures for different food matrices is no longer necessary.
mericon DNA Bacteria Kit and mericon DNA Bacteria Plus Kit
To date, the predominant method to test food for the presence of pathogens has been via culture preparation and/or ELISA-based assays. Molecular-based methods, specifically real-time PCR, offer an informative complement to these well-established methods. In addition, real-time PCR detection delivers results in less time, affords greater sensitivity and specificity, and has the robustness to cope with difficult food matrices. Finally, real-time PCR is a safe method for detecting challenging pathogen strains.
Procedure
DNeasy mericon Food Kit
The optimized protocols for the DNeasy mericon Food Kit use CTAB in combination with Proteinase K to first digest compact tissue and then to subsequently precipitate proteins with simultaneous precipitation of other cellular and food-derived inhibitors.
Inhibitors are precipitated by centrifugation, while the extracted DNA remains in solution. In the subsequent chloroform extraction, any remaining CTAB-protein, CTAB-debris, or CTAB-polysaccharide complex not precipitated is removed together with other lipophilic inhibitors by extraction into the organic chloroform phase. Only the aqueous phase containing the DNA and significantly depleted inhibitors is processed further. This phase is mixed with binding buffer (to adjust binding conditions) and applied to the QIAquick Spin Columns. The resulting DNA is immediately ready for use in a downstream mericon real-time PCR assay.
mericon DNA Bacteria Kit and mericon DNA Bacteria Plus Kit
To provide a streamlined and straightforward method for microbial sample preparation from pathogen enrichment cultures, QIAGEN has designed protocols based on mechanical lysis (using beads) and thermal lysis (by boiling) that deliver optimal disruption force for different bacteria types.
The thermal lysis procedure using the mericon DNA Bacteria Kit is optimized for the majority of easily lysed Gram-negative bacteria (e.g., Salmonella spp., Camplylobacter spp., and Cronobacter spp.), whereas the advanced features of the mechanical sample preparation using the mericon DNA Bacteria Plus Kit create the lysis conditions needed for difficult Gram-positive bacteria, such as Listeria spp.
Carefully formulated chemistry included in the mericon DNA Bacteria Kit and the mericon DNA Bacteria Plus Kit maximizes lysis efficiency, stabilizes the extracted DNA, and removes inhibitors that may interfere with downstream applications.
Developed to be combined with mericon PCR Assays, both sample preparation systems deliver a DNA suspension derived from pathogen enrichment cultures, suitable for sensitive real-time PCR analysis.
Customer reviews
No reviews were found for Mericon - DNA Bacteria Kit - For Extraction Of DNA From Bacteria After Enrichments. Be the first to review!