Print-ELISA/Immunoprinting-ELISA - Model 3DF1+3CA5 Monoclonal Antibodies - Universal CTV Detection
From Viruses and Viroids
10 nitrocellulose membranes (7×13 cm) with pre-printed positive and negative citrus tissue controls. Bovine sero albumin (BSA) as blocking agent. CTV-specific monoclonal antibodies 3DF1+3CA5 alkaline phosphatase conjugated. PBS buffer x10 concentrated. Mineral salts and Tween-20 for washing buffer preparation. Sigma-Fast tablets of precipitin substrate (BCIP+NBT) for alkaline phosphatase. Detailed protocol.