Model PVA - Suspicious Potato Plant Samples Test Strip
From Agri Strip- Potatoes
This test is intended to detect PVA in suspicious potato plant samples. PVA is found in most potato growing areas. The virus may lower yield slightly. In combination with other viruses, it can cause severe diseases (depending on varieties) and yield reduction. Aphids are responsible for virus spread.
Specificity and sampling instruction
Three monoclonal antibodies are used for this AgriStrip assay. One of them was developped against a Swiss isolate from «Advira» and 2 different antibodies raised against a Scottish isolate from «Red Pontiac». This AgriStrip assay reacts specifically with PVA. No crossreaction was observed with other potato viruses, such as PVS, PVX, PVM, PVY, and PLRV. Strongest coloration of testbands is obtained with an 1:60 (w/v) dilution of an extract of infected potato leaves.
Instructions for use
- Place approx. 0.1 g of leaf (corresponds to the size of 1 Euro coin or ≈ 5 cm2) into an extraction bag (Fig. 1*) and add 3 ml of AgriStrip extraction buffer B with a disposable pipette (1:30 w/v).
- Homogenize the tissue with a handheld homogenizer with a few movements for not more than 2-3 seconds (Fig. 2*).
- Transfer 2 drops of extract (Fig. 3*) and 2 drops of extraction buffer into a cuvette.
- Insert the end of the strip marked «sample» into the extract (Fig. 4*) and observe formation of colored bands.