REAGEN LLC

- Model RNM98001 - Aflatoxin M1 ELISA Test Kit

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Aflatoxin m1 elisa kit RNM98001 Product Description REAGEN™ Aflatoxin m1 elisa kit is a Competitive Enzyme immunoassay for the Quantitative Analysis of Aflatoxin M1 in Milk and Milk Powder. The unique features of the kit are: • No need to extract the sample. The sample can be directly used for the ELISA. • High sensitivity (0.05 ng / g or ppb) and low detection limit in milk (0.1ppb) • High reproducibility • A quick ELISA assay (30 minutes ELISA reaction) Sample Type Detection Limit (ng/g or ppb) Milk 0.005 Milk powder 0.005 (corresponding to the dissolved liquid milk; 1:10) Cheese 0.05 Yogurt/Butter milk/Soured milk 0.01 Spec:96 T Shelf life:12 months

The unique features of the kit are:

  • No need to extract the sample. The sample can be directly used for the ELISA
  • High sensitivity (0.05 ng/g or ppb) and low detection limit in milk(0.1ppb).
  • High reproducibility.
  • A quick ELISA assay(30 minutes ELISA reaction).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with capture antibodies directed against anti-Aflatoxin M1 antibodies. Aflatoxin M1 standards or sample solution, Aflatoxin M1–HRP conjugate and anti-Aflatoxin M1 antibodies are added. Free Aflatoxin M1 and Aflatoxin M1-HRP Conjugate compete for the Aflatoxin M1 antibody binding sites. At the same time, the anti- Aflatoxin M1 antibodies are also bound by the immobilized capture antibodies. Any unbound Aflatoxin M1-HRP Conjugate is then removed in a washing step. The TMB Substrate is added to the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

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