- Livestock Vaccine - Brucella Rose Bengal Plate Test Antigen



Ros-Bendoll is a killed standardised suspension of Brucella abortus S 99 strain stained by rose Bengal dye. Indications and Contraindications: It is used for serologic diagnosis of brucellosis by detecting Immunoglobulins induced by smooth Brucella species (B. abortus, B. melitensis and B. suis) in blood serum of humans and animals. False positive reactions may be observed due to nonspecific reactions (like natural antibodies) or cross reacted antibodies generated by vaccine strains or unrelated bacterial species like Y. enterocolitica 09, Salmonella spp. E.coli spp. Test antijen can not detect antibodies resulted from infection caused by rough brucella strains like B.canis, B.ovis, B.abortus RB51. Because this test a screening test, all the positive samples by Ros-Bendoll should also be tested for confirmation.

Composition per dose:

  • Inactivated B.abortus S99 bacterial suspension: 8 %
  • Phenol: 0.45 %
  • Rose-Bengal: 0.03 %

Precautions: Serum samples which are hemolyzed, contaminated with bacterial and chemical agents should not be tested because these factors might effect test performance. Care should be taken not to contact test antigen with eye and hand. Shake the antigen bottle well before use without making bubbles. For the test, a drop of antijen should be mixed with equal volume of serum samples and results should be evaluated according to the agglutination.

Packing: 2.5 ml, 5 ml and 10 ml of antigen in glass bottles with calibrated droppers (40 µl), test plate and stirring stick.
Storage: At +2oC/+8oC and protected from light. Do not freeze.

Destruction conditions: All used bottles should be disposed of in accordance with local requirements of Medicinal Wastes Control Legislation.

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