Model S (PVS) - Suspicious Potato Plant Samples Test Strip
From Agri Strip- Potatoes
This test is intended to detect PVS in suspicious plant samples. It causes few or no symptoms and is carried by many cultivated potato varieties and decreases the yield of potato tubers by up to 20%. Infected potato leaves may show slight chlorosis, roughness of the surface and undulation of the margin.
Specificity and sampling instruction
The antibodies used for this AgriStrip assay were made against a potato isolate of PVS and specifically react with PVS. Sensitivity was comparable to the DAS-ELISA format and strongest coloration of testbands was obtained with a 1:60 to 1:120 (w/v) dilution of an extract of infected potato leaves.
Instructions for use
- Place approx. 0.1 g of leaf (corresponds to the size of 1 Euro coin or ≈ 5 cm2) into an extraction bag (Fig. 1*) and add 3 ml of AgriStrip extraction buffer A with a disposable pipette (1:30 w/v).
- Homogenize the tissue with a handheld homogenizer with a few movements for not more than 2-3 seconds (Fig. 2*).
- Transfer 2 drops of extract (Fig. 3*) and 2 drops of extraction buffer into a cuvette.
- Insert the end of the strip marked «sample» into the extract (Fig. 4*) and observe formation of colored bands.