Belgis Biotech Laboratories

PRE Basic Potato Seeds Production Services

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In order to develop the production of high quality potato seeds , it is primordial to supply the head of the multiplication chain with vegetal material of very high sanitary quality. The use of in vitro micro-propagation techniques can satisfy this objective.
In that way, to the demand of Belgian or foreign producers, the in vitro laboratory can :

  • Supply initial plant material of ‘market free’ potato varieties, under the form of vitroplants, vitrotubers or minitubers. If the demand concerns protected varieties, the client must obtain all the authorisations of the maintainer or the obtainer. This material will be supplied with all the needed guarantees regarding the phyto-sanitary status.
  • Maintain, exclusively for the client, his own varieties or clones and make them available when needed.
  • Introduce, in vitro, plant material from tubers and if necessary apply the adapted techniques to guarantee the phyto-sanitary status of this clone or variety (thermo-therapy and meristem extraction and raising).

Material

The in vitro laboratory of the Farming Systems Section has a collection of more than 350 potato varieties. The phyto-sanitary status of
each of them has been strictly checked: common viruses (PLRV-PVY-PVX-PVS-PVA-PVM), quarantine viruses (PVV, PVT, APLV, APMV, AVB, PBRSV, PYV, TRV, PMTV, TSWV), viroïd (PSTVd), quarantine bacteria (Clavibacter michiganensis subsp. sepedonicus, Ralstonia solanacearum) and common bacteria (Erwinia chrysanthemi, Erwinia carotovora subsp. atroseptica).

Methodology

The in vitro introduction is performed from tubers : several sprout nodes are removed, disinfected and placed on Murashige and Skoog medium enriched with sugar and solidified with Agar. Each node is individualized as a clone until the obtention of all the results concerning its sanitary status. A single healthy clone is choosen to be introduced in the in vitro collection. The control analyses are carried out, for virus and viroïd detection, on leaves of plants raised during a minimum of 4 weeks in greenhouse and, for bacteria, on vitroplants. Clones or varieties are maintained, in the laboratory, under a photoperiod of 16 h light and 8 h dark, at 18 °C.
If needed, the laboratory is able to proceed to the elimination of viruses diseases by a thermo-therapy followed by an extraction and raising of meristematic cells.
The in vitro multiplication is carried out from the vitroplants stored in the lab. An additional and complete phytosanitary control is made during the multiplication phase.
The minitubers production is carried out by transferring the vitroplants to the greenhouse in a hydroponic system : an additional sanitary control is applied on the produced minitubers (common viruses, common and quarantine bacterial diseases).
The microtubers production is carried out in the lab in vitroplants containers. The microtuber induction is completed by adding sugar and specific hormones in the culture media and by placing the containers in the dark at 20°C. The phytosanitary controls are executed on acclimatized vitroplants before the microtuberization.
The production of rooted and acclimatized plantlets is carried out through the transfer of the vitroplants in compressed clods of peat. The acclimatization and rooting phase take place in the greenhouse during 4 weeks before the transfer in the field. An additional sanitary control is carried out on leaves taken on the plants just before their transfer (common viruses) or on the base of a sample of plants (common and quarantine bacterial diseases).

A certificate of initial material delivery (F0 for vitroplants or microtubers or rooted acclimatized plants – F1 for minitubers) as well as the phyto-sanitary passport (EEC) or phyto-sanitary certificate (Third Countries) are delivered to the client with the material.

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