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Sampling for plant pathogen analyses


Selecting sick plants

For good diagnosis we would like to have complete plants with clear symptoms. We prefer both plants that are in an early stage of infection and plants that are seriously infected. For a good diagnose and to increase the chance of detecting the pathogen we would like to have 3 till 6 plants. For a good comparison it also helps to send a healthy plant. This can help especially with plants that are not common or plants of which the infection is difficult to see.

When you send only roots or leaves there is a change that we do not detect the primary pathogen. Therefore we always ask to send in whole plants. If a whole plant is too big to send then you can send roots, stem base, top and parts with symptoms. When you send in samples with post take care that the pant and soil are separated. Pack the plant in paper to avoid that the plant starts to rot.

Sampling procedure pest and diseases

Sampling nematodes

You can take nematode samples the same way as you take soil samples for chemical analyses. You can choose to sample the whole plot or a diseased spot. Nematodes can be present deep in the soil therefore you have to sample upon 50 cm depth.

Sampling for DNA-Previscan

You can take the water sample for a DNA-previscan the same way as you take a normal water sample. If you want to sample a specific place then you can take the water from around that specific place.

Germination count

It is very important to work hygienically when you sample for a germination count. A few drops of dirty water, a non-sterile bottle or touching the bottle with dirty hands can cause false results. Always disinfect the tap and let the water flow for 10 seconds. Disinfect your hands or work with gloves when taking a sample and put the sample in a sterile bottle. 

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