MilliporeSigma

Analysis of Aflatoxin B1, B2, G1, and G2 in Corn Meal, Wheat Meal, and Raw Peanut Paste Matrices

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Courtesy of MilliporeSigma

The Supel Tox AflaZea SPE cartridge was used to demonstrate the cleanup of corn meal, wheat meal, and raw peanut paste matrices prior to the analysis of Aflatoxin B1, B2, G1, and G2. In the procedure, corn meal, wheat meal, and raw peanut paste extracts were spiked with Aflatoxin Standard Mix (Product No. 34036) and run through the Supel Tox AflaZea SPE cartridges. The eluate was collected and diluted for HPLC analysis.

Figure 1. Results From Cleanup using Supel Tox AflaZea SPE Cartridges

HPLC Conditions
column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm (Product No. 53823-U); mobile phase: (A) water, (B) acetonitrile, (C) methanol (700:120:120,A:B:C) with 0.780 g potassium bromide and 230 µL nitric acid; flow rate: 0.400 mL/min; column temp.: 35 °C; detector: Fluorescence detector; Excitation: 360 nm; Emission: 440 nm
injection: 40 µL

Results illustrate that Supel Tox AflaZea SPE cartridges provide exceptional cleanup of corn meal, wheat meal, and raw peanut paste samples, yielding high analyte recoveries and low %RSD for aflatoxin analysis.

Figure 2. Analyte Recovery and %RSD of Aflatoxins from Corn, Wheat, and Peanut Paste (n = 3)

In the “bind and elute” strategy, the analyte of interest becomes bound to the SPE sorbent. The unwanted matrix is removed, and the analyte is ultimately eluted. In “interference removal,” the unwanted matrix components remain bound to the sorbent, while the purified analylte is eluted. This strategy reduces the number of purification steps, allowing for time savings and increase in sample throughput. The Supel Tox AflaZea, DON, and Tricho SPE cartridges employ the “interference removal” strategy.

Figure 3. Comparison of 'Bind and Elute' and 'Interference' Principles

Comparison of Supel Tox SPE Cartridges to Immunoaffinity Columns for Matrix Removal Prior to Mycotoxin Analysis

  • Ten times faster = Ten times sample throughput
  • Vast improvement in reproducibility

An experiment comparing the use of the Supel Tox AflaZea SPE cartridge to a leading immunoaffintiy column for the analysis of Aflatoxins B1, B2, G1, and G2 in peanut paste, illustrates that the Supel Tox AflaZea SPE cartridge is superior to the immunoaffinity purification methods in terms of process simplicity, time required for sample preparation, and control of variation.

Table 1. Procedure for Analysis of Aflatoxin (n = 3) using Immunoafinity vs. Supel Tox

Figure 4. Cleanup of Aflatoxins in Peanut Paste: Immunoaffinity Versus Supel Tox (n=3)

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