Application of a GPC-LC-MS/MS method for the determination of 31 mycotoxins in edible oils

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Gel Permeation Chromatography (GPC) is widely used for sample clean up in mycotoxin analysis. The most commonly described methods use GPC columns packed with SX-3 BioBeads suitable for cleaning Zearalenone, Aflatoxins and Trichothesenes from edible oils and fatty matrices. Separation of Fumonisins from the oil fraction are inadequate with this column.

The new GPC column designed by LCTech shows superior performance for simultaneous clean up of Zearalenone, Trichothesenes (types A, B and D), Aflatoxins, Ochratoxin A, including Fumonisins, along with other Mycotoxins from edible oil.

Method Development
Different column materials and compositions have been tested for their suitability to separate the analytes from the oil fraction. Influence of the eluent composition, pH, temperature and the column capacity had been investigated. The selected material with optimized parameters was tested by a direct GPC-MS/MS coupling. The separation of the analytes from the oil was satisfactory up to oil concentration of 0.1 g/mL THF (fig1).

Influence of Water Content
Variation of the water content of the eluent had a remarkable influence on the retention times of the fumonisins but had little effect on other toxins. Eluent containing 10 % of water provided good separation (fig. 2) but increasing water content to 20 % caused coelution of fumonisins with the oil fraction.

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