John Wiley & Sons, Ltd.

Copper induced root growth inhibition of Allium cepa var. Agrogarum L. involves disturbances in cell division and DNA damage

0
Copper (Cu) is considered an indispensable microelement for plants. However, excessive Cu is toxic and disturbs several processes in the plant. The present study addressed the effects of ionic Cu (2.0 µM and 8.0 µM) on mitosis, microtubule cytoskeleton and DNA in root tip cells of Allium cepa var. agrogarum L., in order to better understand Cu toxicity on plant root systems. Results indicated that Cu was accumulated in roots and that root growth was inhibited dramatically in Cu treatment groups. Chromosomal aberrations (e.g., C‐mitosis, chromosome bridges, chromosome stickiness, and micronucleus) were observed and the mitotic index decreased during Cu treatments at different concentrations. Microtubules (MTs) were one of the target sites of Cu toxicity in root tip meristematic cells and the exposure to Cu substantially impaired MTs arrangements. The content of α‐tubulin decreased following 36 h of exposure to 2.0 or 8.0 µM of Cu in comparison with the control group. Cu increased DNA damage and suppressed cell cycle progression. The above toxic effects got more serious with increasing Cu concentration and prolonging exposure time. This article is protected by copyright. All rights reserved

Customer comments

No comments were found for Copper induced root growth inhibition of Allium cepa var. Agrogarum L. involves disturbances in cell division and DNA damage. Be the first to comment!