Boston Bioproducts, Inc. products

Non-fat powdered milk is a widely utilized blocking agent in laboratory applications such as Western blotting, immunohistochemistry (IHC), and enzyme-linked immunosorbent assays (ELISA). Its primary function is to prevent non-specific binding of antibodies to proteins by serving as a buffering agent during both primary and secondary antibody incubations. This helps in reducing overall background noise in experimental results. However, it should not be used as a blocking agent when dealing with phosphorylated proteins due to its phosphoprotein content, which might interfere with results and increase background. It's essential for laboratories to choose the appropriate blocking agent depending on the target antigen involved. Used effectively, non-fat powdered milk helps in achieving more accurate and cleaner results in various biochemical assays. Availability in customizable sizes allows for flexible usage in different experimental settings.