AA3002 Determination of Trace Lead, Poly-Phenols and Tannins in Wines Using a Single Analytical Instrument - Application Notes

Determination of Trace Lead,Poly-Phenols and Tannins in WinesUsing a Single Analytical InstrumentThe manufacturing of wine and otherfermented beverages has typically been afairly simple process. Historically, theexpertise of the sage winemaster was usedto gauge the quality of the product. Today,many environmental concerns, health factors,and government rules, prompt the producersof fine wines to monitor several importantcomponents in the grape must, thefermentation vats, and in the final wine.Typically, several different analyticalinstruments have been used to perform thesetests including Flame Atomic AbsorptionSpectrophotometers (FAAS), UV Spectro-photometers (UV), and Colorimeters (VIS).The 210VGP Atomic Absorption systemfrom Buck Scientific, Inc. employs a highperformance monochromator and a uniquecell-holding attachment that allow it to beused as three analytical tools in one: FAAS,UV and VIS. This means that three of themore critical tests can be performed with asingle device. The FAAS system normallyuses a Lead Hollow Cathode Lamp (HCL)to measure the Lead signal at 283nm, whilethe UV test for Poly-Phenols at 280nm isbest done with a D2 (Deuterium) HCL. TheVIS determination of Tannins at 520nm usesa Cr (Chromium) HCL and the Buck #5711cell holder. Tannins can also be measured at760nm using a Cs (Cesium) HCL.Other testprocedures for measurements at 420nm and620nm can be done with a Rb (Rubidium)HCL. Examples of actual samples are listedon the back.Analyst: Gerald J. DeMennaB U C KScientificApplication Note#AA3002A11/95 SIC: 2084Basic Conditions: (for best linearity and precision)Lead Calibration: 5 ppm Standard and an Acetic Acid BlankPhenol Calibration: 2.0, 5.0, and 10 ppm in 5% Acetic Acid; 10 cm. QuartzTannin Calibration: 25, 100, and 500 ppm in Water: Ethanol; 5 cm. GlassSample Pb Phenols TanninsID (ppm) (ppm) (ppm)Napa VarietalMust 8.7 768 2,570Napa VarietalFirst Ferment 6.3 227 982Medocino CountySavignon Blanc 0.93 169 64LaCour PavillionBordeaux 1.4 403 1,328The ultimate flexibility of the Buck 210VGP design permits this unique testing capability to be done very economicallyand efficiently with just one system, saving space, money, and time.Basic System: $12,950.00Turnkey System: $16,107.00For detailed configuration, refer to Quote #AA4002AIncludes: All recommended lamps, standards, and accessories fornormal operation.1-800-562-556658 Fort Point Street l East Norwalk, CT 06855Phone: 203-853-9444 l Fax: 203-853-0569quality@bucksci.com l www.bucksci.comB U C KScientificDetermination of Trace Lead,Poly-Phenols and Tannins in WinesUsing a Single Analytical InstrumentThe manufacturing of wine and otherfermented beverages has typically been afairly simple process. Historically, theexpertise of the sage winemaster was usedto gauge the quality of the product. Today,many environmental concerns, health factors,and government rules, prompt the producersof fine wines to monitor several importantcomponents in the grape must, thefermentation vats, and in the final wine.Typically, several different analyticalinstruments have been used to perform thesetests including Flame Atomic AbsorptionSpectrophotometers (FAAS), UV Spectro-photometers (UV), and Colorimeters (VIS).The 200A Atomic Absorption systemfrom Buck Scientific, Inc. employs a highperformance monochromator and a uniquecell-holding attachment that allow it to beused as three analytical tools in one: FAAS,UV and VIS. This means that three of themore critical tests can be performed with asingle device. The FAAS system normallyuses a Lead Hollow Cathode Lamp (HCL)to measure the Lead signal at 283nm, whilethe UV test for Poly-Phenols at 280nm isbest done with a D2 (Deuterium) HCL. TheVIS determination of Tannins at 520nm usesa Cr (Chromium) HCL and the Buck #5711cell holder. Tannins can also be measured at760nm using a Cs (Cesium) HCL.Other testprocedures for measurements at 420nm and620nm can be done with a Rb (Rubidium)HCL. Examples of actual samples are listedon the back.Analyst: Gerald J. DeMennaB U C KScientificApplication Note#AA3002B11/95 SIC: 2084Basic Conditions: (for best linearity and precision)Lead Calibration: 5 ppm Standard and an Acetic Acid BlankPhenol Calibration: 2.0, 5.0, and 10 ppm in 5% Acetic Acid; 10 cm. QuartzTannin Calibration: 25, 100, and 500 ppm in Water: Ethanol; 5 cm. GlassSample Pb Phenols TanninsID (ppm) (ppm) (ppm)Napa VarietalMust 8.7 768 2,570Napa VarietalFirst Ferment 6.3 227 982Medocino CountySavignon Blanc 0.93 169 64LaCour PavillionBordeaux 1.4 403 1,328The ultimate flexibility of the Buck 200A design permits this unique testing capability to be done very economically andefficiently with just one system, saving space, money, and time.Basic System: $8,495.00Turnkey System: $11,652.00For detailed configuration, refer to Quote #AA4002BIncludes: All recommended lamps, standards, and accessories fornormal operation.1-800-562-556658 Fort Point Street l East Norwalk, CT 06855Phone: 203-853-9444 l Fax: 203-853-0569quality@bucksci.com l www.bucksci.comB U C KScientific