Mycotoxin Standards Brochure

Mycotoxin StandardsSingle- and Multi-Component Standard Solutions13C - Isotopically LabeledStandardsMatrix Certified Reference MaterialsDried Down StandardsvSome fungal genera like Aspergillus, Fusarium, Penicillium, etc. produce mycotoxins. These toxic second-ary metabolites are harmful to animal and human health if they enter the food chain via contaminated crops and fruits or through processed food and feed products. Therefore, in at least 100 countries limits are set for mycotoxin levels for food and feed. For a precise detection of all regulated mycotoxins, Sigma-Aldrich offers a comprehensive range of standards, including mixture solutions, 13C isotopically labeled standards, certified reference materials (CRM) and dried down standards.Find our products at sigma-aldrich.com/mycotoxinssigma-aldrich.com/ mycotoxinsFor accurate and reliable results we currently offer 5 mg neat reference materials with certified concentrations for various mycotoxins. Try our reference materials for the calibration of your analytical instruments and recovery experiments.Mycotoxin reference materials (Neats)Catalog No.Component Package [mg]32927 3-Acetyldeoxynivalenol 532928 15-Acetyldeoxynivalenol 532754 Aflatoxin B1532755 Aflatoxin B2532756 Aflatoxin G1532757 Aflatoxin G2532943 Deoxynivalenol 532936 Fumonisin B15Catalog No.Component Package [mg]33438 Fusarenon-X 532932 Neosolaniol 532929 Nivalenol Hydrate 532937 Ochratoxin A 532759 Patulin 532609 Sterigmatocystin 533947 T-2 Toxin 532939 Zearalenone 5For precise quality control of food and feed, we offer single component standard solutions of the most important mycotoxins in various concentra-tions and different solvents.Mycotoxin single component standard solutionsCatalog No. Component Con. [µg/g] Solvent Package [mL]34132 3-Acetyldeoxynivalenol 100 Acetonitrile 234133 15-Acetyldeoxynivalenol 100 Acetonitrile 234129 Acetyl-Deoxynivalenol-D3100 Acetonitrile 234029 Aflatoxin B12 Acetonitrile 246323-U Aflatoxin B13 Benzene/Acetonitrile (98/2) 1 x 5 5 x 144647-U Aflatoxin B120 Methanol 146324-U Aflatoxin B23 Benzene/Acetonitrile (98/2) 134034 Aflatoxin B20.5 Acetonitrile 234032 Aflatoxin G12 Acetonitrile 246325-U Aflatoxin G13 Benzene/Acetonitrile (98/2) 134033 Aflatoxin G20.5 Acetonitrile 246326-U Aflatoxin G23 Benzene/Acetonitrile (98/2) 134031 Aflatoxin M10.5 Acetonitrile 246319-U Aflatoxin M110 Acetonitrile 135405 Alpha-Zearalanol 10 Acetonitrile 135406 Alpha-Zearalanol 10 Acetonitrile 135407 Beta-Zearalanol 10 Acetonitrile 135409 Beta-Zearalanol 10 Acetonitrile 1Continuevsigma-aldrich.com/ mycotoxinsCatalog No. Component Con. [µg/g] Solvent Package [mL]35410 Citrinin 100 Acetonitrile 146911 Deoxynivalenol 200 Ethylacetate/Methanol (95/5) 134155 Deoxynivalenol-D1100 Acetonitrile 234135 Deepoxy-deoxynivalenol 50 Acetonitrile 234124 Deoxynivalenol 100 Acetonitrile 232911 Deoxynivalenol-3-glucoside 50 Acetonitrile 134137 Diacetoxyscirpenol 100 Acetonitrile 237017 Fumagillin 100 Acetonitrile 134139 Fumonisin B150 Acetonitrile/Water 234142 Fumonisin B250 Acetonitrile/Water 232606 Fumonisin B350 Acetonitrile/Water 134130 Fusarenon-X 100 Acetonitrile 235598 Gliotoxin 100 Acetonitrile 134136 HT-2 Toxin 100 Acetonitrile 237013 Moniliformin 100 Acetonitrile/Water 134138 Neosolaniol 100 Acetonitrile 234131 Nivalenol 100 Acetonitrile 234037 Ochratoxin A 10 Acetonitrile 246912 Ochratoxin A 50 Benzene/Acetic Acid (99/1) 132411 Ochratoxin B 10 Acetonitrile 234127 Patulin 100 Acetonitrile 246914-U Patulin 100 Chloroform 135417 Paxilline 100 Acetonitrile 132986 Sterigmatocystin 50 Acetonitrile 132914 T-2 Tetraol 50 Acetonitrile 134071 T-2 Toxin 100 Acetonitrile 232913 T-2 Triol 50 Acetonitrile 137016 Verruculogen 100 Acetonitrile 135441 Wortmannin 100 Acetonitrile 146916-U Zearalenone 50 Acetonitrile 134126 Zearalenone 100 Acetonitrile 2Fully 13C-isotope labeled mycotoxin standards (e.g. Aflatoxin B1-13C17; Figure 1) are stable, non-radioactive isotopically labeled versions of the target analyte with the same physiochemical and chromatographic behaviors. These internal standards provide an accurate detection, as they show an optimal mass unit difference between the analyte and the standard, which prevents interference. They also account for variations during sample preparation, cleanup and ionization. Sigma-Aldrich offers labeled Aflatoxins (B1, B2, G1 and G2), Fumosins (B1, B2, B3), Deoxynivale-nol and six other mycotoxin standards.13C-isotopically labeled internal standards solutions for LC-MSCatalog No. Description Con. [µg/mL] Solvent Package [mL]32962 3-Acetyldeoxynivalenol-13C1725 Acetonitrile 132764 Aflatoxin B1-13C170.5 Acetonitrile 132771 Aflatoxin B2-13C170.5 Acetonitrile 1Continuevsigma-aldrich.com/mycotoxinsSigma-Aldrich offers some reference materials as dried down standards which are packaged in amber ampoules to ensure stability. The convenient reconstitution of these standards, made possible by the addition of an appropriate solvent, is described in the enclosed certificate of analysis.Dried down mycotoxin standards (RMs)Catalog No. Component Con. [µg/g] Package [mL]35758 Alternariol 100 0.135762 Alternariol-9-methyl ether 100 0.137012 Beauvericin (BEA) 100 0.1For a detailed analysis of individual mycotoxins in multitoxin samples, we offer nine mixtures of mycotoxin groups which often occur naturally in various proportions in food and feed samples.Mycotoxin mixture standard solutions for multi-analyte detectionCatalog No. Description Components Con. [µg/mL] Solvent Package [mL]46300-U Aflatoxin Mix B1, G1B2, G213Benzene/ Acetonitrile (98/2)5x146303 Aflatoxin Mix B1, G1B2, G213Methanol 1x546304-U Aflatoxin Mix B1, G1B2, G213Methanol 5x134036 Aflatoxin Mix 4 B1, G1B2, G220.5Acetonitrile 1, 233415 Aflatoxin Mix 4 B1, G1, B2, G220 (each) Acetonitrile 240139-U Japanese Aflatoxin Mix B1, G1, B2, G225 (each) Acetonitrile 5x134143 Fumonisin Mix FB1, FB250 (each) Acetonitrile/Water 232926 Trichothecene Mix 3-AcDON, DON,NIV, FusX, HAT-2,T-2, DAS, ZON10 (each) Acetonitrile 134134 B-Trichothecen Mix DON, NIV, 3-AcDON, 15-AcDON100 Acetonitrile 2Catalog No. Description Con. [µg/mL] Solvent Package [mL]32772 Aflatoxin G1-13C170.5 Acetonitrile 132777 Aflatoxin G2-13C170.5 Acetonitrile 132488 Chloramphenicol-13C1125 Acetonitrile 1.232489 Cyclosporine A-13C6225 Acetonitrile 1.234128 Deoxynivalenol-13C1525 Acetonitrile 133621 Fumonisin B1-13C3425 Acetonitrile/Water 132915 Fumonisin B2-13C3410 Acetonitrile/Water 132916 Fumonisin B3-13C3410 Acetonitrile/Water 132491 Griseofulvin-13C1725 Acetonitrile 1.233842 HT-2 Toxin-13C2225 Acetonitrile 132773 Mycophenolic acid-13C1725 Acetonitrile 133416 Ochratoxin A-13C2010 Acetonitrile 135516 Patulin-13C725 Acetonitrile 1.233892 T-2 Toxin-13C2425 Acetonitrile 132758 Zearalenone-13C1825 Acetonitrile 113C13C13C13C13C13C13C13C13CO13C13C13C13C13CO13C 13COOOH313COHHFigure 1. Molecular structure of a fully 13C-isotope labeled Aflatoxin B1-13C17Continuevsigma-aldrich.com/mycotoxinsThe certified mycotoxin reference standard solutions from IRMM provide an accurate determination of detection limits as well as validation of analytical methods. These standards are certified according to the ISO Guides 34 and 35 as described in the enclosed certificate. All mycotoxins are dissolved in acetonitrile and solutions are placed in 4 mL amber glass ampoules. We offer Aflatoxin M1 dissolved in chloroform.Mycotoxin solutions (CRMs)Catalog No. Component Con. [µg/g] Solvent Package [mL]ERMAC057 Aflatoxin B13.79 Acetonitrile 4ERMAC058 Aflatoxin B23.80 Acetonitrile 4ERMAC059 Aflatoxin G13.78 Acetonitrile 4ERMAC060 Aflatoxin G23.80 Acetonitrile 4IRMM315 4-Deoxynivalenol 25.1 Acetonitrile 4IRMM316 Nivalenol 24.0 Acetonitrile 4Con. [µg/ml]BCR423RM Aflatoxin M19.93 Chloroform 2.5ERMAC699 Zearalenone 9.95 Acetonitrile 4Catalog No. Component Con. [µg/g] Package [mL]CRMs being released from IRMM and distributed by Sigma-Aldrich are produced with raw materials to more accurately resemble actual samples in their natural state. For performance control of mycotoxin analysis we offer compound feed, maize, wheat and peanut butter reference materials.Certified matrix reference materials for mycotoxin analysis (CRMs)Catalog No. Product name Component Con. [µg/kg] Package [g]BCR375 Compound Feed (Aflatoxin blank) Aflatoxin B1<1 50ERMBE375 Compound Feedingstuff (Aflatoxins, very low level) Aflatoxin B12.6 2x75Aflatoxin B20.20Aflatoxin G10.4Aflatoxin G2<0.2ERMBE376 Compound Feedingstuff (Aflatoxins, high level) Aflatoxin B112.9 2x75Aflatoxin B20.68Aflatoxin G15.2ERMBC716 Maize (Zearalenone, very low level) Zearalenone <5 60ERMBC717 Maize (Zearalenone, low level) Zearalenone 83 60BCR377 Maize Flour (Deoxynivalenol, blank) Deoxynivalenol <50 150BCR401R Peanut Butter (Aflatoxin, low level) Aflatoxin B1<0.2 100Aflatoxin B2(each)Aflatoxin G1Aflatoxin G2BCR471 Wheat (Ochratoxin A, blank) Ochratoxin A <0.6 5535878 Citreoviridin A 100 0.135970 Meleagrin 100 0.137025 Retrorsine 50 0.0535976 Stachybotrylactam 100 0.135977 Tentoxin 100 0.137018 Tenuazonic acid 100 0.1vOYD10/2012ArgentinaFree Tel: 0810 888 7446Tel: (+54) 11 4556 1472Fax: (+54) 11 4552 1698AustraliaFree Tel: 1800 800 097 Free Fax: 1800 800 096Tel: (+61) 2 9841 0555Fax: (+61) 2 9841 0500AustriaTel: (+43) 1 605 81 10Fax: (+43) 1 605 81 20BelgiumFree Tel: 0800 14747Free Fax: 0800 14745Tel: (+32) 3 899 13 01Fax: (+32) 3 899 13 11BrazilFree Tel: 0800 701 7425Tel: (+55) 11 3732 3100Fax: (+55) 11 5522 9895CanadaFree Tel: 1800 565 1400Free Fax: 1800 265 3858Tel: (+1) 905 829 9500Fax: (+1) 905 829 9292ChileTel: (+56) 2 495 7395Fax: (+56) 2 495 7396 ChinaFree Tel: 800 819 3336Tel: (+86) 21 6141 5566Fax: (+86) 21 6141 5567Czech RepublicTel: (+420) 246 003 200Fax: (+420) 246 003 291DenmarkTel: (+45) 43 56 59 00Fax: (+45) 43 56 59 05FinlandTel: (+358) 9 350 9250Fax: (+358) 9 350 92555FranceFree Tel: 0800 211 408Free Fax: 0800 031 052Tel: (+33) 474 82 28 88Fax: (+33) 474 95 68 08GermanyFree Tel: 0800 51 55 000Free Fax: 0800 64 90 000Tel: (+49) 89 6513 0Fax: (+49) 89 6513 1160HungaryIngyenes telefonszám: 06 80 355 355Ingyenes fax szám: 06 80 344 344Tel: (+36) 1 235 9063Fax: (+36) 1 269 6470IndiaTelephoneBangalore: (+91) 80 6621 9400New Delhi: (+91) 11 4358 8000Mumbai: (+91) 22 2570 2364Hyderabad: (+91) 40 4015 5488Kolkata: (+91) 33 4013 8003FaxBangalore: (+91) 80 6621 9650New Delhi: (+91) 11 4358 8001Mumbai: (+91) 22 4087 2364Hyderabad: (+91) 40 4015 5488Kolkata: (+91) 33 4013 8000IrelandFree Tel: 1800 200 888Free Fax: 1800 600 222Tel: (+353) 402 20370Fax: (+ 353) 402 20375IsraelFree Tel: 1 800 70 2222Tel: (+972) 8 948 4100Fax: (+972) 8 948 4200ItalyTel: (+39) 02 3341 7310Fax: (+39) 02 3801 0737JapanTel: (+81) 3 5796 7300Fax: (+81) 3 5796 7315KoreaFree Tel: (+82) 80 023 7111Free Fax: (+82) 80 023 8111Tel: (+82) 31 329 9000Fax: (+82) 31 329 9090MalaysiaTel: (+60) 3 5635 3321Fax: (+60) 3 5635 4116MexicoFree Tel: 01 800 007 5300Free Fax: 01 800 712 9920Tel: (+52) 722 276 1600Fax: (+52) 722 276 1601The NetherlandsFree Tel: 0800 022 9088Free Fax: 0800 022 9089Tel: (+31) 78 620 5411Fax: (+31) 78 620 5421New ZealandFree Tel: 0800 936 666Free Fax: 0800 937 777Tel: (+61) 2 9841 0555Fax: (+61) 2 9841 0500NorwayTel: (+47) 23 17 60 00Fax: (+47) 23 17 60 10PolandTel: (+48) 61 829 01 00Fax: (+48) 61 829 01 20PortugalFree Tel: 800 202 180Free Fax: 800 202 178Tel: (+351) 21 924 2555Fax: (+351) 21 924 2610RussiaTel: (+7) 495 621 5828Fax: (+7) 495 621 5923SingaporeTel: (+65) 6779 1200Fax: (+65) 6779 1822SlovakiaTel: (+421) 255 571 562Fax: (+421) 255 571 564 South AfricaFree Tel: 0800 1100 75Free Fax: 0800 1100 79Tel: (+27) 11 979 1188Fax: (+27) 11 979 1119SpainFree Tel: 900 101 376Free Fax: 900 102 028Tel: (+34) 91 661 99 77Fax: (+34) 91 661 96 42SwedenTel: (+46) 8 742 4200Fax: (+46) 8 742 4243SwitzerlandFree Tel: 0800 80 00 80Free Fax: 0800 80 00 81Tel: (+41) 81 755 2828Fax: (+41) 81 755 2815United KingdomFree Tel: 0800 717 181Free Fax: 0800 378 785Tel: (+44) 1747 833 000Fax: (+44) 1747 833 313United StatesToll-Free: 800 325 3010Toll-Free Fax: 800 325 5052Tel: (+1) 314 771 5765Fax: (+1) 314 771 5757VietnamTel: (+84) 3516 2810Fax: (+84) 6258 4238Internetsigma-aldrich.comOrder/Customer Service (800) 325-3010 • Fax (800) 325-5052 Technical Service (800) 325-5832 • sigma-aldrich.com/techserviceDevelopment/Custom Manufacturing Inquiries (800) 244-1173Safety-related Information sigma-aldrich.com/safetycenterSigma-Aldrich Worldwide Offi ces©2012 Sigma-Aldrich Co. 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