Recovery of high value protiens from dairy fluids technology update

23-May-03Recovery of High Value Proteins from Dairy FluidsTechnology UpdateSixth Symposium on Advances in Dairy Products Technology“Dairy Ingredients Symposium” Shell Beach, CAMarch 1-2, 2004Functional ingredientsMilk natural and transgenicEgg white/egg yolkPlant extracts, natural and transgenicFermentation broth, natural and GMOAnimal tissue extracts Animal blood and plasma- Proteins - Peptides - Enzymes -- Immunoglobulins - Hormones -- Inhibitors - Polyphenols - Nucleic acids -- Polysaccharides - LipidsIsolation of Bio-active SubstancesRaw materialMULTIPLE STEPSCentrifugationFiltration/MicrofiltrationUltrafiltrationPrecipitationDiafiltrationEvaporation/DistillationConcentrationChromatographyFinal ProductSweet Whey Protein FractionsImmunoglobulinLactoferrin and LactoperoxidaseBSAß-lactoglobulina-lactoalbuminGMPMol. Wt.15,000 –1,000,00075,00069,0004,000-40,00018,30014,000Concentration in Whey, %Total 0.610 %0.300 %0.070 %0.140 %0.030 %0.010 %0.060 %Properties of Whey Proteins• ß-Lactoglobulin: Major whey protein. Gelation, foam, emulsification• a-Lactoalbumin: Major protein in human milk. Infant formula, emulsification.• Immunoglobulin: Colostrums substitute.• Bovine Serum Albumin: Lipid binding, infant formula.• Lactoferrin: Microbial inhibitor, Infant formula, immune modulator?Application examples Bovine whey proteins Infant formulaNon-allergenicproductsToothpasteFood productsFunctional food NutraceuticalNutritional productsMedicalproductsNutritionalbarsSports andperformanceproductsHealth careproductsShampoos VaccinesWound healingProcess Outline for Chromatographic SeparationResin ready for useLoadingRegenerationWashingElutionProtein Fraction MF UF DryingExpanded Bed (EBA) Vs Packed BedAgarose – tungsten carbide conglomerateDensity 2.5-3.5 g/ml Size range 20-200 micronExpanded Bed AdsorbtionLoad Wash ElutionTarget molecules are retainedUnwanted material is washed awayTarget molecules are releasedExpanded Bed (EBA) Vs Packed BedExpanded Bed (EBA)• Small beads • Minimum pretreatment• Reduced clogging• Feed distribution critical• Carry over ?• Higher throughputPacked Bed• Large beads• Pre-filtration essential• Back flushing ?• Limited by pressure dropLactoferrin from Sweet wheyEquilibration with waterLoad with sweet whey1 LF Standard2 Raw whey3 Run through4 Wash with saline buffer (LP)5 Eluate (LF)Wash waterWash with BufferElution, pH gradientConcentration of EluateDrying1 2 3 4 5 Properties of Whey Proteins Expanded Bed (EBA) Vs Packed Bed Expanded Bed Adsorbtion Expanded Bed (EBA) Vs Packed Bed Lactoferrin from Sweet whey