Model PN 53012B -Aflatoxin Plate Kit

ASSAY PRINCIPLES
The Aflatoxin kit is a competitive enzyme-labeled immunoassay. Aflatoxin is extracted from a ground sample by blending or shaking with methanol/water. The extract is then diluted with water, filtered and then tested in the immunoassay. Aflatoxin-HRP enzyme conjugate is pipetted into the test wells followed by calibrators or sample extracts. Aflatoxin antibody is then pipetted into the test wells to initiate the reaction. During the 10 minute incubation period, aflatoxin from the sample and aflatoxin-HRP enzyme conjugate compete for binding to aflatoxin antibody which, in turn, binds to the test well. Following this 10 minute incubation, the contents of the well are removed and the wells are washed to remove any unbound toxin or enzyme-labeled toxin. A clear substrate is then added to the wells and any bound enzyme-toxin conjugate causes the conversion to a blue color. Following a 10 minute incubation, the  reaction is stopped and amount of color in each well is read. The color of unknown samples is compared to the color of the calibrators and the Aflatoxin concentration of the samples is derived.