Model PN 53014B -Fumonisin Plate Kit

ASSAY PRINCIPLES
The Fumonisin kit is a competitive enzyme-labeled immunoassay. Fumonisin is extracted from a ground sample by shaking with methanol/water. The extract is then filtered and the extract is tested in the immunoassay. Fumonisin-HRP enzyme conjugate is pipetted into the test wells followed by calibrators or sample extracts. Fumonisin antibody is then pipetted into the test wells to initiate the reaction. During the 10 minute incubation period, fumonisin from the sample and fumonisin-HRP enzyme conjugate compete for binding to fumonisin antibody which, in turn, binds to the test well. Following this 10 minute incubation, the contents of the well are removed and the wells are washed to remove any unbound enzyme-labeled toxin. A clear substrate is then added to the wells and any bound enzyme-toxin conjugate causes the conversion to a blue color. Following a 5 minute incubation, the reaction is stopped and amount of color in each well is read. The color of unknown samples is compared to the color of the calibrators and the Fumonisin concentration of the samples is derived.

SPECIFICITY
The antibody utilized in the Abraxis Fumonisin Plate Kit is specific for fumonisins. The calibrators are formulated with fumonisin B1 The following table shows the relative reactivity for other forms: