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VSIModel Q-NF1LP - Nitrogen Fixation Package

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Q-NF1LP Nitrogen Fixation Package, the only complete experimental package for non-destructively measuring nitrogen fixation in H2-producing legume symbioses.

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The low range flow-through H2 sensor (Q-S121) measures the rate of H2 production from N2-fixing tissues, allowing in vivo approximation of nitrogenase activity in real time. The package also includes the Q-S151 CO2 analyser (0-2000 ppm), which is used to measure nodulated root respiration. The Q-NF1LP package contains everything needed for successful experiments, including gas exchange pots for growing legumes such as soybeans under nodule-producing conditions, nutrient solution recipes, rhizobia inoculum, and detailed instructions. When combined with the Q-GSS Gas Switching System, nitrogenase activity can be measured in up to 3 legume root systems in parallel (plus a control channel; open flow setting). The N fixation package contains everything needed to carry out a wide range of laboratory studies on the physiology and biochemistry of N2 fixation in an open-flow gas exchange system. The analysers are easy to calibrate, set-up time is minimal and experiments are simple to perform. Applications of the Q-NF1LP range from studies of the effects of temperature/photosynthetic assimilation on nitrogenase activity and root respiration, N fixation efficiency of different genotypes, inhibition of N2 fixation by fertilisation, to the electron allocation coefficient of nitrogenase, etc.

  • Non-invasive measurements of nitrogenase activity and respiration from nodulated roots
    • Flow-through gas analysis system for measuring nitrogenase activity in H2-evolving rhizobial symbioses
    • Parallel measurement of CO2 evolution from nodulated roots
  • Easy set-up and calibration
  • Software calculations of nitrogen fixation rates
  • Multiplexing system, 4 chambers (optional)
  • Custom experimental pots (size, material) available