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pEASY - Model CE111-01 -Blunt E1 Expression Kit
pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.
- 5 minutes fast ligation of Pfu-amplified PCR products.
- Ampicillin resistance.
- T7 promoter primer and T7 terminator primer for sequencing.
- Bacteriophage T7lac promoter for high level expression.
- N-terminal 6×His tag for easy purification.
- Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
- E1 Expression Plasmid included as negative control.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70? for six months; Others at -20? for nine months.
Shipping
Dry ice (-70?).
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