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BCRModel F-Spores - Phenotypically Engineered Bacterial Spores

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F-spores are phenotypically engineered bacterial spores that function as self-reporters of germination (PLoS ONE 5, e9283, 2010). F-spores have a unique property of being fluorogenic, i.e., they are non-fluorescent when dormant, but produce intense fluorescence a few minutes after the onset of germination.

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Biological Indicators – e.g., BCR-30-min BI

Biological indicators (BIs) are test systems used to monitor sterilization processes in health care facilities. BIs contain spores of heat-resistant bacteria, e.g. Geobacillus stearothermophilus. After sterilization, the number of surviving spores is measured. There are many different commercial BIs with a broad range of performance and cost. The CDC regulates the use of BIs in healthcare facilities.

LEXSAS (label-free exponential signal-amplification system)

Biosensing system for detecting low levels of bacteria in real-time. The system exploits the F-spore’s ability to produce fluorescence when sensing a germinant. The LEXSAS has been used for identifying bacterially contaminated platelet concentrates used for transfusion, because the system offers rapid analysis, high sensitivity, and low cost https://www.ncbi.nlm.nih.gov/pubmed/12480543.