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UniTab - Model AMM -Ammonia Reagent
Ammonia UniTab Reagent (=AMM): An enzymatic test kit for determination of ammonia in grape juice, fermenting wine, and other liquid samples. Contains enzymes, substrates and buffer components in tablet form and as aqueous solutions and suspensions.
Primary Amino Nitrogen UniTab Reagent (=PAN): Determine primary amino nitrogen compounds (or Nitrogen by OPA = NOPA) in grape juice & fermenting wine/must samples.
Contains buffered N-Acetyl-l-cysteine (NAC) tablets, ophthaldialdehyde (OPA), and Nitrogen Standard Solution.
Each tablet is dissolved in 5 mL of deionized water, permitting the user to prepare only as much working reagent as required for daily use. This “fast blanking” method only requires working reagent (the blank reagent solution is no longer required). This procedure is based on a published method from UC Davis.
Why Measure Yeast Assimilable Nitrogen compounds (Ammonia Nitrogen + Primary Amino Nitrogen)? Typical total YAN ranges from 40 – 560mg/L; both yeast & M-L Bacteria require available nitrogen for effective fermentation.
- Low Ammonia Nitrogen & PAN levels have been associated with sluggish fermentation and sensory imperfections.
- Nitrogen content may be supplemented e.g. with diammonium phosphate (DAP.)
- Routine and excessive nitrogen supplementation should be avoided, since residual nitrogen supports the growth of unwanted wine spoilage organisms such as Brettanomyces, Lactic Acid Bacteria, Acetobacter, etc.