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GEN-IAL - Genetically Modified Organisms (GMOs)
EU foodstuffs produced from GMO products or containing GMO products have been subject to mandatory labelling since September 1, 1998. According to EU Regulation 1829/2003, labeling is required by law for food and animal feed with a GMO content of 0.9% or more. In order to find out whether a product is genetically modified, a real-time PCR screening procedure is used to test for gene sequences (NOS, 35S, PAT etc.) that do not occur naturally in plants. This method is used to detect the most common GMO plants. For new GMO plants on the market that are not detected by this screening, the PCR methods are continuously adapted to the needs of the market. GMO positive screening samples are tested for the event in question in the next step and checked for approval in Europe. If both criteria are met, the GMO content must be quantified. If the GMO content exceeds 0.9%, the food must be labelled as GMO positive. The analytical parameters must be adjusted for “GMO-free” labelling.
- Raw materials: e.g. soybeans, grain, …
- Food: e.g. baby food, baked goods, delicatessen, soy drinks, …
- Highly processed products: e.g. starch, lecithin, soy sauce, tomato puree, cereals, …
- Beverage bases: e.g. concentrates, fruit nectar, …
- Animal feed: e.g. pet food, concentrated feed, …
The Multiplex GenControl PCR-Kits enable the qualitative and quantitative detection of genetically modified organisms in different materials and include screening and event-specific kits. They have been developed and validated in accordance with German (§64 LFGB, LAG) and European standards (DIN, CEN, ISO) and are continuously tested in ring trials and proficiency tests. The systems are sensitive, specific and easy to use.
Detection is carried out by means of fluorescence measurement using the hydrolysis probe format (TaqMan®). Using hot-start PCR plus double-labelled sequence-specific probes (FAM / HEX / ROX / CY5), a measurable fluorescence signal of a defined wavelength is emitted during the extension phase if hybridization to the target sequence is correct. An inhibition control is amplified together with the specific sequence in a reaction tube to exclude false negative results due to inhibition.