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- VSI - Model SP-4020 - Shoot PhenoBox
VSI - Model SP-4020 -Shoot PhenoBox
The Phenobox is a open hardware phenotyping system (Czedik‐Eysenberg et al. 2018) for the assessment of shoot traits of small to medium-sized plants (not rosette). The 'PhenoBox' itself consists of an imaging chamber that autonomously captures and processes the shoot images from different perspectives. The hardware is complemented by 'PhenoPipe', an open source analytical framework for sample management and evaluation.The standard Shoot Phenobox SP-4020 has the following external dimensions: 75.5 cm high, 50 cm wide and 82 cm (side) + 4 cm (door handle) long. It weighs approximately 15 kg. The Imaging area is suitable for plants with a maximum shoot size of approximately 20 cm width and 40 cm height. We are happy to discuss the manufacture of custom Shoot Phenoboxes with other dimensions (e.g. for plants with higher/wider canopies).
The camera can be adjusted in height to achieve a centered position on the shoot for imaging. Homogeneous illumination of the imaging area is provided by 7 LED panels, two on each side of the imaging axis (vertical) and three (horizontal) above the imaging compartment. Any camera compatible with gphoto2 can be used in the Phenobox; in the standard Phenobox, a Canon SLR camera (700D) is installed for easy operation and high image quality. Focus can be set automatically or manually (i.e. recommended setting for smaller shoots). The system is controlled by a Rasberry Pi 3 microcomputer.
Pots are positioned with a pot holder on a rotational stand; entry point is through the front door (standard) or custom side or top doors/lids. The included pot holder has two sides for square pot sizes with 4.5 or 5.5 cm edges and various heights (standard); custom pot holders (for round pots, etc.) can be manfactured to meet the required experimental conditions. Pots are labeled with QR codes for automatic plant registration in the PhenoPipe server system (see below). Label printer & software (suitable for setting up experimental schemes) included. The standard Phenobox comes with 6 preset rotation angles for imaging each plant shoot, ranging from 0° to 300° in 60° increments (absolute positions). To set custom rotation angles, see the GitHub documentation on the motor controller or discuss a different factory preset with us.
