Phenotyping Articles
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Production of dryland barley for human food: quality and agronomic performance
Grain β-glucan content is the most important attribute for barley (Hordeum vulgare L.) varieties destined for the human food market. This trait is important because of the blood glucose and cholesterol-reducing properties of β-glucans. High levels of grain protein content, test weight, and seed size and endosperm color may also add value. Seed yield potential, in part, determines the ...
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QTL analysis of root architecture traits and low Phosphorus tolerance in an Andean bean population
Tolerance to low P soils is a desirable trait in common bean (Phaseolus vulgaris L.) cultivars grown in acid-weathered soils. Genetic variability in response to P-deficient soils exists in the Andean gene pool. G19833, a low P–tolerant indeterminate Andean landrace, has been evaluated for quantitative trait loci (QTL) and tolerance to low P in combination with Mesoamerican parents. Our goal was ...
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Genetic basis of the low-Phytate trait in the soybean line CX1834
The low-phytate (LP) trait in plant seeds offers important nutritional and environmental benefits for food and feed uses. Mutants with reduced phytate content are commonly produced by chemical mutagenesis, as is the case for soybean [Glycine max (L.) Merr.] line CX1834. Quantitative trait loci (QTL) for LP in CX1834 have previously been reported, however the genetic basis for this trait has not ...
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Genetic mapping of sheath blight resistance QTLS within tropical Japonica rice cultivars
Most commercial cultivars of rice (Oryza sativa L.) are susceptible to sheath blight (SB), a devastating fungal disease causing significant losses in grain yield and quality. There are limited sources of genetic resistance adapted to U.S. growing conditions, and no commercial long grain cultivar of rice is currently available in the United States with a high level of SB resistance. Sheath blight ...
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Optimization of the marker-based procedures for pyramiding genes from multiple donor lines: III. multiple-gene assemblage using background marker selection
Constructing high-degree gene-pyramided lines has important practical implications; such lines could be used for multiple purposes, for example, as a high-powered breeding stock line, a material line for characterizing multigene interactions, or a market variety as it stands. Effectiveness of two typical marker-based schemes for constructing such lines, named AF (gene assemblage first) and BF ...
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Gas exchange and transpiration ratio in Sorghum
Decreased water availability and increased food demand worldwide require development of more water-efficient crops. This study was conducted to examine preflower transpiration ratio (CO2 assimilation rate [A]/transpiration rate [E], A:E) in four inbred sorghum [Sorghum bicolor (L.) Moench] lines and 12 of their F1 hybrids in terms of phenotypic and genetic variation, inheritance (i.e., additive ...
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Heritability of drought resistance traits and correlation of drought resistance and agronomic traits in peanut
Inheritance of traits is important for developing effective breeding schemes for improving desired traits. The aims of this study were to estimate the heritabilities (h2) of drought resistance traits and the genotypic (rG) and phenotypic (rP) correlations between drought resistance traits and agronomic traits, and to examine the relationships between drought resistance traits under stressed and ...
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Identification of QTL for resistance to Sclerotinia stem rot in soybean plant introduction 194639
Sclerotinia stem rot of soybean [Glycine max (L.) Merr.], caused by Sclerotinia sclerotiorum (Lib.) de Bary, is a difficult disease to manage, although some gains have been made through breeding for quantitative resistance. The objective of the present study was to map quantitative trait loci (QTL) controlling partial resistance to Sclerotinia stem rot from the soybean plant introduction (PI) ...
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Mutations in a Δ 9–Stearoyl-ACP-Desaturase gene are associated with enhanced stearic acid levels in soybean seeds
Stearic acid (18:0) is typically a minor component of soybean [Glycine max (L.) Merr.] oil, accounting for only 2 to 4% of the total fatty acid content. Increasing stearic acid levels of soybean oil would lead to enhanced oxidative stability, potentially reducing the need for hydrogenation, a process leading to the formation of undesirable trans fatty acids. Although mutagenesis strategies have ...
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Response of heterozygous and homozygous Imidazolinone-resistant spring wheat genotypes to Imazamox
Imidazolinone-resistant (IR) spring wheat (Triticum aestivum L.) was released commercially in Canada in 2004, providing growers with a new opportunity to selectively control a broad range of weeds in wheat, including volunteer cereals and annual grasses. Imidazolinone (IMI) herbicides inhibit acetolactate synthase in susceptible (S) plants, but IR wheat plants have an altered target site that ...
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Translation initiation factor 2 mutant alters start codon selection independent of Met-tRNA binding
Selection of the AUG start codon for translation in eukaryotes is governed by codon-anticodon interactions between the initiator Met-tRNAiMet and the mRNA. Translation initiation factor 2 (eIF2) binds Met-tRNAiMet to the 40S ribosomal subunit, and previous studies identified Sui– mutations in eIF2 that enhanced initiation from a noncanonical UUG codon, presumably by impairing Met-tRNAiMet ...
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Phosphorylated ssk1 prevents unphosphorylated ssk1 from activating the ssk2 mitogen-activated protein kinase kinase kinase in the yeast high-osmolarity glycerol osmoregulatory pathway
In Saccharomyces cerevisiae, external high osmolarity activates the Hog1 mitogen-activated protein kinase (MAPK), which controls various aspects of osmoadaptation. Ssk1 is a homolog of bacterial two-component response regulators and activates the Ssk2 MAPK kinase kinase upstream of Hog1. It has been proposed that unphosphorylated Ssk1 (Ssk1-OH) is the active form and that Ssk1 phosphorylated ...
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The crucial role of the pls1 tetraspanin during ascospore germination in podospora anserina provides an example of the convergent evolution of morphogenetic processes in fungal plant pathogens and saprobes{triangledown}
Pls1 tetraspanins were shown for some pathogenic fungi to be essential for appressorium-mediated penetration into their host plants. We show here that Podospora anserina, a saprobic fungus lacking appressorium, contains PaPls1, a gene orthologous to known PLS1 genes. Inactivation of PaPls1 demonstrates that this gene is specifically required for the germination of ascospores in P. ...
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Different genetic functions for the rpd3(l) and rpd3(s) complexes suggest competition between nua4 and rpd3(s)
Rpd3(L) and Rpd3(S) are distinct multisubunit complexes containing the Rpd3 histone deacetylase. Disruption of the GCN5 histone acetyltransferase gene shows a strong synthetic phenotype when combined with either an sds3 mutation affecting only the Rpd3(L) complex or an rco1 mutation affecting only Rpd3(S). However, these synthetic growth defects are not seen in a gcn5 sds3 rco1 triple mutant, ...
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Aspergillus nidulans arfb plays a role in endocytosis and polarized growth
Filamentous fungi undergo polarized growth throughout most of their life cycles. The Spitzenkörper is an apical organelle composed primarily of vesicles that is unique to filamentous fungi and is likely to act as a vesicle supply center for tip growth. Vesicle assembly and trafficking are therefore important for hyphal growth. ADP ribosylation factors (Arfs), a group of small GTPase proteins, ...
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Leishmania adaptor protein-1 subunits are required for normal lysosome traffic, flagellum biogenesis, lipid homeostasis, and adaptation to temperatures encountered in the mammalian host
The adaptor protein-1 (AP-1) complex is involved in membrane transport between the Golgi apparatus and endosomes. In the protozoan parasite Leishmania mexicana mexicana, the AP-1 µ1 and 1 subunits are not required for growth at 27°C but are essential for infectivity in the mammalian host. In this study, we have investigated the function of these AP-1 subunits in order to understand the molecular ...
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Regulation of histone h4 lys16 acetylation by predicted alternative secondary structures in rox noncoding rnas
Despite differences in size and sequence, the two noncoding roX1 and roX2 RNAs are functionally redundant for dosage compensation of the Drosophila melanogaster male X chromosome. Consistent with functional conservation, we found that roX RNAs of distant Drosophila species could complement D. melanogaster roX mutants despite low homology. Deletion of a conserved predicted stem-loop structure in ...
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Unconventional genomic architecture in the budding yeast saccharomyces cerevisiae masks the nested antisense gene nag1
The genomic architecture of the budding yeast Saccharomyces cerevisiae is typical of other eukaryotes in that genes are spatially organized into discrete and nonoverlapping units. Inherent in this organizational model is the assumption that protein-coding sequences do not overlap completely. Here, we present evidence to the contrary, defining a previously overlooked yeast gene, NAG1 (for nested ...
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Involvement of saccharomyces cerevisiae avo3p/tsc11p in maintaining tor complex 2 integrity and coupling to downstream signaling
Target-of-rapamycin proteins (TORs) are Ser/Thr kinases serving a central role in cell growth control. TORs function in two conserved multiprotein complexes, TOR complex 1 (TORC1) and TORC2; the mechanisms underlying their actions and regulation are not fully elucidated. Saccharomyces TORC2, containing Tor2p, Avo1p, Avo2p, Avo3p/Tsc11p, Bit61p, and Lst8p, regulates cell integrity and actin ...
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Ercc1-xpf endonuclease facilitates dna double-strand break repair
ERCC1-XPF endonuclease is required for nucleotide excision repair (NER) of helix-distorting DNA lesions. However, mutations in ERCC1 or XPF in humans or mice cause a more severe phenotype than absence of NER, prompting a search for novel repair activities of the nuclease. In Saccharomyces cerevisiae, orthologs of ERCC1-XPF (Rad10-Rad1) participate in the repair of double-strand breaks (DSBs). ...
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