Paralytic shellfish toxins dinoflagelate-derived neurotoxins in bivalve mollusks


Courtesy of Pickering Laboratories, Inc.

Analysis Of Dinoflagelate Derived Neurotoxins In Bivalve Mullusks Using Hplc Postcolumn Fluorescence Method
The paralytic shellfish toxins are a group of 18 secondary metabolites deposited in bivalve mollusks by dinoflagelates. Dinoflagelates blooms are seasonal, occurring during warm months. Since it is unpredictable whether an infestation will occur, the shellfish popula¬tion should be regularly monitored for toxins. Ingestion of contaminated shellfish can lead to paralytic shellfish poisoning; a life-threat¬ening illness.

Mouse bioassay is the official method of AOAC International, but the drawbacks associated with this method have led to exploration of chemical methods. The most common HPLC post-column method is to oxidize the separated toxins under alkaline conditions to a fluorescent compound. Sullivan et al. used this method to determine the gonyautoxins 1-6 (GTX1-6), saxitoxin (STX) and neosax- itoxin (neoSTX) but not the N-sulfocarbamoyl-11-hydroxysulfate toxins (C1-C4). Oshima et al.2,3 modified this method to determine the 3 toxin groups separately using isocratic elution with 3 different mobile phases. Further improvement by Jeffery van de Riet et al.4> 5 has led to a shorter analysis time to determine the 3 groups of toxins using step gradient and a switching valve. This method abstract describes the use of Pickering Laboratories Pinnacle PCX post-column derivatization system for the HPLC post-column determina¬tion of paralytic shell fish toxins.

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